CRISPR Essentials: CRISPR gRNAs, Cas9 and Related Products

CRISPR Formats

^§ Modified tracrRNA is needed when using modified crRNA.

SygRNA^® synthetic CRISPR two-part system requires both crRNA and tracrRNA to complex with Cas9. When ordering sgRNA or crRNA:tracrRNA, be sure to select the species that matches your Cas9 source.

View our protocols and frequently asked questions to learn more about SygRNA^® synthetic CRISPR.  

Bacteria Glycerol Stocks: provided in Terrific Broth supplemented with carbenicllin (100 µg/mL) and 10% glycerol

For Sanger QuickPick^™ Knockout gRNAs or custom gRNAs

Learn more about Sanger QuickPick Screening Libraries and Clones
 

No. of Clones Ordered	Techware	Volume	Product Availability
1-24	Individually labeled vials	500 µL	Ships in 2-3 days
25+	96 well plates	120 µL	Ships in 3-10 business days

Plasmid DNA: provided in 1xTris-EDTA

For Sanger QuickPick^™ Knockout gRNAs or custom gRNAs

Learn more about Sanger QuickPick Screening Libraries and Clones
 

Quantity of DNA ordered	Concentration	Techware	Volume	Product Availability– Sanger QuickPick™ Knockout Clones	Product Availability– Custom CRISPR Clones
1 µg	20 ng/µL	1-24 clones: individually labeled vials 25+ clones: 96 well plates	50 µL	Ships in 3 weeks; additional time for clone quantities > 100	Ships in 4 weeks; additional time for clone quantities > 100
10 µg 25 µg 100 µg	Concentration will be reported with product	individually labeled vials	Varies depending on concentration	Ships in 3-4 weeks; additional time for clone quantities > 25	Ships in 4-5 weeks; additional time for clone quantities > 25

Lentivirus: provided in Dulbecco's Modified Eagle's Medium supplemented with 10% Fetal Bovine Serum and 1% Penicillin/Streptomycin

For Sanger QuickPick™ Knockout gRNAs or custom gRNAs

Learn more about Sanger QuickPick Screening Libraries and Clones

Web ordered lentivirus clones are provided in individually labeled vials. Requests for aliquots in plates should be submitted to CRISPR@sial.com.

Volume	Titer
	106	107	108	109
100 µL	N/A	N/A	2 x 50 µL 4 x 25 µL	5 x 20 µL
200 µL	1 x 200 µL 2 x 100 µL 4 x 50 µL 8 x 25 µL	1 x 200 µL 2 x 100 µL 4 x 50 µL 8 x 25 µL	4 x 50 µL 8 x 25 µL	10 x 20 µL
1 mL	5 x 200 µL 10 x 100 µL 20 x 50 µL 40 x 25 µL	5 x 200 µL 10 x 100 µL 20 x 50 µL 40 x 25 µL	20 x 50 µL 40 x 25 µL	50 x 20 µL
2 mL	10 x 200 µL 20 x 100 µL 40 x 50 µL 80 x 25 µL	10 x 200 µL 20 x 100 µL 40 x 50 µL 80 x 25 µL	40 x 50 µL 80 x 25 µL	100 x 20 µL
5 mL	25 x 200 µL 50 x 100 µL 100 x 50 µL 200 x 25 µL	25 x 200 µL 50 x 100 µL 100 x 50 µL 200 x 25 µL	100 x 50 µL 200 x 25 µL	N/A

Sanger QuickPick™ Knockout gRNA Lentivirus Manufacturing Timelines

Please allow for additional shipping time
 

Volume	Titer
	106	107	108	109
100 µL	N/A	N/A	5 weeks	5 weeks
200 µL	4 weeks	4 weeks	5 weeks	5 weeks
1 mL	5 weeks	5 weeks	5 weeks	5 weeks
2 mL	5 weeks	5 weeks	5 weeks	5 weeks
5 mL	5 weeks	5 weeks	5 weeks	N/A

MISSION™ CRISPR gRNA Lentivirus Manufacturing Timelines

Please allow for additional shipping time

Volume	Titer
	106	107	108	109
100 µL	N/A	N/A	6 weeks	6 weeks
200 µL	5 weeks	5 weeks	6 weeks	6 weeks
1 mL	6 weeks	6 weeks	6 weeks	6 weeks
2 mL	6 weeks	6 weeks	6 weeks	6 weeks
5 mL	6 weeks	6 weeks	6 weeks	N/A

In Vitro Transcribed RNA (IVT): provided in Molecular Grade Water (DNase-, RNase-, Protease-free)
 

Techware	Volume & Concentration	Product Availability
Individually labeled vials	20 µL, 200 ng/µL	Ships in 5 weeks; allow additional time for clone quantities > 25.

Cas9 Products

Click on the product number to order and see additional product details
 

Cas9 Proteins

Product No.	Product	Description/Application
CAS9GFPPRO	Wild-type SpCas9-EGFP Fusion – NEW!	Original Wild-Type Cas9 fused to enhanced green fluorescent protein. Ideal for flow cytometry applications and visualization of transfected RNP complexes. Highly efficient
ECAS9GFPPR	Enhanced specificity SpCas9-EGFP Fusion – NEW!	Cas9 variant with Improved specificity fused to enhanced green fluorescent protein. Ideal for flow cytometry applications, fluorescence microscopy and for experiments that require high accuracy
CAS9PROT	Wild-type SpCas9	Original , wild-type Cas9; simple and economical; efficient in a broad range of gene editing applications
ESPCAS9PRO	Enhanced specificity SpCas9	Cas9 variant with improved specificity ideal for experiments that require high accuracy
CAS9D10APR	Cas9D10A Nickase	A two-gRNA system with improved specificity and decreased off-target potential for specialized genome-editing applications
DCAS9PROT	dCas9-3XFLAG™ Biotin	Catalytically inactive SpCas9 protein containing a 3XFLAGTM tag and Biotin; ideal for DNA detection and isolation and is a component of the proxy-CRISPR method
FNCAS9PROT	FnCas9 Protein	Ideal for synthetic biology or cell-free applications

 

Cas9 Plasmids and Lentivirus
 

Product No.	Description	Format	Selection Method
CAS9P	SpCas9 plasmid	Plasmid DNA
CAS9GFPP	SpCas9 GFP Plasmid	Plasmid DNA	Fluorophore (GFP)
CAS9RFPP	SpCas9 RFP Plasmid	Plasmid DNA	Fluorophore (RFP)
CAS9GEMP	SpCas9 GFP Geminin Plasmid	Plasmid DNA	Fluorophore (GFP)
CAS9D10AP	SpCas9-D10A Nickase Plasmid	Plasmid DNA
CAS9D10AGFPP	SpCas9-D10A Nickase GFP Plasmid	Plasmid DNA	Fluorophore (GFP)
CAS9D10ARFPP	SpCas9-D10A Nickase RFP Plasmid	Plasmid DNA	Fluorophore (RFP)
DCAS9P	SpdCas9 Plasmid	Plasmid DNA
DCAS9P300	SpdCas9-p300 Activator Plasmid	Plasmid DNA	Fluorophore (GFP)
DCAS9P300RFP	SpdCas9-p300 RFP Activator Expression Plasmid	Plasmid DNA	Fluorophore (RFP)
FNCAS9P	FnCas9 Plasmid	Plasmid DNA
CAS9MRNA	SpCas9 mRNA	mRNA
CAS9D10AMRNA	SpCas9-D10A Nickase mRNA	mRNA
CAS9BST	SpCas9 Blasticidin Lenti Plasmid	Plasmid DNA	Blasticidin
CAS9HYGROVP	SpCas9 Hygromycin Lenti Plasmid	Plasmid DNA	Hygromycin
CAS9NEO	SpCas9 Neomycin Lenti Plasmid	Plasmid DNA	Neomycin
CAS9ZEOVP	SpCas9 Zeo Lenti Plasmid	Plasmid DNA	Zeo
CAS9GFPVP	SpCas9 GFP Lenti Plasmid	Plasmid DNA	Fluorophore (GFP)
CAS9RFPVP	SpCas9 RFP Lenti Plasmid	Plasmid DNA	Fluorophore (RFP)
ESPCAS9BST	High Fidelity Sp Cas9 Blasticidin Lenti Plasmid	Plasmid DNA	Blasticidin
LVCAS9BST	SpCas9 Blasticidin Lenti Particles	Lentivirus	Blasticidin
LVCAS9NEO	SpCas9 Neomycin Lenti Particles	Lentivirus	Neomycin
CAS9BAC1P	SpCas9 Lambda Red Homologous Recombination Plasmid for E.coli	Plasmid DNA	Ampicillin

 

SAM CRISPR Activator Cas9 Products

 

Product No.	Description	Compatible gRNA vector	System	Format	Selection Method	Comment
SAMHELPERV	SAM CRISPRa Helper Construct Kit Lentiviral Transduction Particles	LV06 & LV07	Lentiviral	Lentivirus	Blasticidin & Hygromycin	Order compatible gRNA separately Kit contains 2 components : • dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 • MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
SAMHELPERP	SAM CRISPRa Helper Construct Kit Plasmid DNA	LV06 & LV07	Lentiviral	Plasmid DNA	Blasticidin & Hygromycin	Order compatible gRNA separately Kit contains 2 components : • dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 • MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
SAMVP64BSTV	dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 Lentiviral Transduction Particles	LV06 & LV07	Lentiviral	Lentivirus	Blasticidin	Order compatible gRNA separately   Requires MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
SAMVP64BST	dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 Plasmid DNA	LV06 & LV07	Lentiviral	Plasmid DNA	Blasticidin	Order compatible gRNA separately   Requires MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
SAMMS2HYGV	MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2 Lentiviral Transduction Particles	LV06 & LV07	Lentiviral	Lentivirus	Hygromycin	Order compatible gRNA separately   Requires dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1
SAMMS2HYG	MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2 Plasmid DNA	LV06 & LV07	Lentiviral	Plasmid DNA	Hygromycin	Order compatible gRNA separately   Requires dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1

Vectors

Mammalian System Vectors
 

Vector No.	Description	Order Clones	Cas9/gRNA Content	Format Availability	Other Features
					Fluorophore	Cas9 type
p01	U6-gRNA:CMV-Cas9-2A-tGFP	p01 clones	Cas9+gRNA	Plasmid DNA	GFP	S. pyogenes
p02	U6-gRNA:CMV-Cas9-2A-tRFP	p02 clones	Cas9+gRNA	Plasmid DNA	RFP	S. pyogenes
p04	U6-gRNA:CMV-eCas9-2a-tGFP	p04 clones	Cas9+gRNA	Plasmid DNA	GFP	enhanced specificity
p03	U6-gRNA	p03 clones	gRNA only	Plasmid DNA

Lentiviral Vectors
 

Vector No.	Description	Order Clones	Cas9/gRNA Content	Format Availability	Selection Method
LV01	U6-gRNA:ef1a-puro-2A-Cas9-2A-tGFP	LV01 clones	Cas9+gRNA	Plasmid DNA & Lentivirus	Fluorophore (GFP) & Puromycin
LV05	U6-gRNA:EF1a-Cas9+FLAG-2A-Puro	LV05 clones†	Cas9+gRNA	Plasmid & Lentivirus	Puromycin
LV06	CRISPRa SAM U6-gRNA:EF1a-Puro	LV06 clones†	CRISPR activator gRNA	Plasmid & Lentivirus	Puromycin
LV07	CRISPRa SAM U6-gRNA:EF1a-Zeo	LV07 clones†	CRISPR activator gRNA	Plasmid & Lentivirus	Zeo
LV04	U6-gRNA:hPGK-puro-2A-tBFP	Sanger QP KO clones†	gRNA only	Plasmid DNA & Lentivirus	Fluorophore (BFP) & Puromycin
LV03	U6-gRNA:ef1a-tGFP	LV03 clones	gRNA only	Plasmid DNA & Lentivirus	Fluorophore (GFP)
LV02	U6-gRNA:ef1a-puro	LV02 clones	gRNA only	Plasmid DNA & Lentivirus	Puromycin

^†Web ordering is currently available in select countries. Global web ordering capabilities are in development. Please contact CRISPR@sial.com for questions or assistance placing clone orders.

Plant Vectors
 

Vector No.	Description	Order Clones	Cas9/gRNA Content	Format Availability	Species	Plasmid Type	Selection
p63	(dicot)U6-gRNA:CMV-Cas9-beta glucuronidase (Agrobacterium plasmid)	p63 clones‖	Cas9+ gRNA	Plasmid DNA	Dicot	Agro-bacterium	Beta Glucuron- idase
p55	(dicot)U6-gRNA:CMV-Cas9-bar (Agrobacterium plasmid)	p55 clones‖	Cas9+ gRNA	Plasmid DNA	Dicot	Agro-bacterium	Biala- phos
p53	(dicot)U6-gRNA:CMV-Cas9-hyg (Agrobacterium plasmid)	p53 clones‖	Cas9+ gRNA	Plasmid DNA	Dicot	Agro-bacterium	Hygro- mycin
p54	(dicot)U6-gRNA:CMV-Cas9-neo (Agrobacterium plasmid)	p54 clones‖	Cas9+ gRNA	Plasmid DNA	Dicot	Agro-bacterium	Neo- mycin
p61	(dicot)U6-gRNA:CMV-Cas9-bar (bioballistic plasmid)	p61 clones‖	Cas9+ gRNA	Plasmid DNA	Dicot	Bio-Ballistic	Biala- phos
p59	(dicot)U6-gRNA:CMV-Cas9-hyg (bioballistic plasmid)	p59 clones‖	Cas9+ gRNA	Plasmid DNA	Dicot	Bio-Ballistic	Hygro- mycin
p60	(dicot)U6-gRNA:CMV-Cas9-neo (bioballistic plasmid)	p60 clones‖	Cas9+ gRNA	Plasmid DNA	Dicot	Bio-Ballistic	Neo- mycin
p62	(monocot)U6-gRNA:CMV-Cas9-beta glucuronidase (Agrobacterium plasmid)	p62 clones‖	Cas9+ gRNA	Plasmid DNA	Monocot	Agro-bacterium	Beta Glucuron- idase
p52	(monocot)U6-gRNA:CMV-Cas9-bar (Agrobacterium plasmid)	p52 clones‖	Cas9+ gRNA	Plasmid DNA	Monocot	Agro-bacterium	Biala- phos
p50	(monocot) U6-gRNA:CMV-Cas9-hyg (Agrobacterium plasmid)	p50 clones‖	Cas9+ gRNA	Plasmid DNA	Monocot	Agro-bacterium	Hygro- mycin
p51	(monocot)U6-gRNA:CMV-Cas9-neo (Agrobacterium plasmid)	p51 clones‖	Cas9+ gRNA	Plasmid DNA	Monocot	Agro-bacterium	Neo- mycin
p58	(monocot)U6-gRNA:CMV-Cas9-bar (bioballistic plasmid)	p58 clones‖	Cas9+ gRNA	Plasmid DNA	Monocot	Bio-Ballistic	Biala- phos
p56	(monocot)U6-gRNA:CMV-Cas9-hyg (bioballistic plasmid)	p56 clones‖	Cas9+ gRNA	Plasmid DNA	Monocot	Bio-Ballistic	Hygro- mycin
p57	(monocot)U6-gRNA:CMV-Cas9-neo (bioballistic plasmid)	p57 clones‖	Cas9+ gRNA	Plasmid DNA	Monocot	Bio-Ballistic	Neo- mycin

^‖ Web ordering is currently available in select countries. For countries where web ordering is not yet available, please fill out the Custom Plant CRISPR Ordering Form

 

Bacterial Editors

Vector No.	Description	Order Clones	Cas9/gRNA Content	Format Availability	Selection
p10	(bacteria) J23119-gRNA-amp	p10 clones	gRNA only	Plasmid DNA	Ampicillin

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CRISPR Controls

Click on the product number to order and see additional product details
 

Product No.	Description	Vector	Control Type	System	Format	gRNA Type	Selection Method
CRISPR06	CRISPR Universal Non-target Negative Control 1, Cas9+gRNA Plasmid	p01	Neg.	Mammalian	Plasmid DNA	Cas9+gRNA	Fluorophore (GFP)
CRISPR07	CRISPR Universal Non-target Negative Control 2, Cas9+gRNA Plasmid	p01	Neg.	Mammalian	Plasmid DNA	Cas9+gRNA	Fluorophore (GFP)
CRISPR08	CRISPR Universal Non-target Negative Control 3, Cas9+gRNA Plasmid	p01	Neg.	Mammalian	Plasmid DNA	Cas9+gRNA	Fluorophore (GFP)
CRISPR17	CRISPR Activator Human Oct4 Positive Control Plasmid, gRNA Only Expression Plasmid	p03	Pos.	Mammalian	Plasmid DNA	gRNA only
CRISPR12	CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lenti Plasmid (vector)	LV01	Neg.	Lentiviral	Plasmid DNA	Cas9+gRNA	Fluorophore (GFP) & Puromycin
CRISPR18	CRISPR-Lenti Universal Non-target Negative Control 1, gRNA Only Lenti Plasmid (Sanger vector)	LV04	Neg.	Lentiviral	Plasmid DNA	gRNA only	Fluorophore (BFP) & Puromycin
CRISPR19	CRISPR-Lenti Universal Non-target Negative Control 2, gRNA Only Lenti Plasmid (Sanger vector)	LV04	Neg.	Lentiviral	Plasmid DNA	gRNA only	Fluorophore (BFP) & Puromycin
CRISPR20	CRISPR-Lenti Universal Non-target Negative Control 3, gRNA Only Lenti Plasmid (Sanger vector)	LV04	Neg.	Lentiviral	Plasmid DNA	gRNA only	Fluorophore (BFP) & Puromycin
CRISPR12V	CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lentiviral Particles (vector)	LV01	Neg.	Lentiviral	Lentivirus	Cas9+gRNA	Fluorophore (GFP) & Puromycin
CRISPR12H	CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lentiviral Particles, High Titer (vector)	LV01	Neg.	Lentiviral	Lentivirus	Cas9+gRNA	Fluorophore (GFP) & Puromycin
CRISPR15H	Gecko2-Lenti Universal Non-Target Negative Control 1, gRNA Only High Titer Lentiviral Particles (Gecko2 vector)	LV02	Neg.	Lentiviral	Lentivirus	gRNA only	Puromycin
CRISPR18V	CRISPR-Lenti Universal Non-target Negative Control 1, gRNA Only Lentiviral Particles (Sanger vector)	LV04	Neg.	Lentiviral	Lentivirus	gRNA only	Fluorophore (BFP) & Puromycin
CRISPR19V	CRISPR-Lenti Universal Non-target Negative Control 2, gRNA Only Lentiviral Particles (Sanger vector)	LV04	Neg.	Lentiviral	Lentivirus	gRNA only	Fluorophore (BFP) & Puromycin
CRISPR20V	CRISPR-Lenti Universal Non-target Negative Control 3, gRNA Only Lentiviral Particles (Sanger vector)	LV04	Neg.	Lentiviral	Lentivirus	gRNA only	Fluorophore (BFP) & Puromycin
CRISPR01	CRISPR Human EMX1 Positive Control Plasmid, gRNA Only Expression Plasmid, includes Cas9 Expression Plasmid (vector)	p03	Pos.	Mammalian	Plasmid DNA	gRNA only
CRISPR02	CRISPR Nickase Human EMX1 Positive Control Plasmids (gRNA Only Expression Plasmids, Includes Cas9-D10A Plasmid)	p03	Pos.	Mammalian	Plasmid DNA	gRNA only
CRISPR11	CRISPR-Lenti Human EMX1 Positive Control, Cas9+gRNA Lenti Plasmid (vector)	LV01	Pos.	Lentiviral	Plasmid DNA	Cas9+gRNA	Fluorophore (GFP) & Puromycin
CRISPR13	CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lenti Plasmid (vector)	LV01	Pos.	Lentiviral	Plasmid DNA	Cas9+gRNA	Fluorophore (GFP) & Puromycin
CRISPR21	CRISPR-Lenti Human HPRT1 Positive Control, gRNA Only Lenti Plasmid (Sanger vector)	LV04	Pos.	Lentiviral	Plasmid DNA	gRNA only	Fluorophore (BFP) & Puromycin
CRISPR11V	CRISPR-Lenti Human EMX1 Positive Control, Cas9+gRNA Lentiviral Particles (vector)	LV01	Pos.	Lentiviral	Lentivirus	Cas9+gRNA	Fluorophore (GFP) & Puromycin
CRISPR13V	CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lentiviral Particles (vector)	LV01	Pos.	Lentiviral	Lentivirus	Cas9+gRNA	Fluorophore (GFP) & Puromycin
CRISPR13H	CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lentiviral Particles, High Titer (vector)	LV01	Pos.	Lentiviral	Lentivirus	Cas9+gRNA	Fluorophore (GFP) & Puromycin
CRISPR16H	Gecko2-Lenti Human HPRT1 Positive Control, gRNA Only High Titer Lentiviral Particles (Gecko2 vector)	LV02	Pos.	Lentiviral	Lentivirus	gRNA only	Puromycin
CRISPR21V	CRISPR-Lenti Human HPRT1 Positive Control, gRNA Only Lentiviral Particles (Sanger vector)	LV04	Pos.	Lentiviral	Lentivirus	gRNA only	Fluorophore (BFP) + Puromycin
CRISPRPL01	CRISPR GUS GAPDH Reporter Control for Monocots	p62	Pos.	Plant	Plasmid DNA	Cas9+gRNA	Beta Glucuronidase
CRISPRPL02	CRISPR GUS GAPDH Reporter Control for Dicots	p63	Pos.	Plant	Plasmid DNA	Cas9+gRNA	Beta Glucuronidase

CRISPR Companion Products

 

HSOLIGOINT	CRISPR Integration Kit - Use to determine baseline for Homology Directed Repair for cell lines
SHP002	CRISPR & MISSION® Lentiviral Packaging Mix
T1706	T1706 TransIT-CRISPR® Transfection Reagent
D6171	Dulbecco’s Modified Eagle’s Medium - high glucose, HEPES modification
D8537	Dulbecco’s Phosphate Buffered Saline
D5796	Dulbecco’s Modified Eagle’s Medium - high glucose P4333 Penicillin-Streptomycin
T3924	Trypsin-EDTA solution
G7513	L-Glutamine solution
CLS430720	Corning® cell culture flasks, 75 cm2
CLS430825	Corning® cell culture flasks, 150 cm2
CLS431082	Corning® cell culture flask, 225 cm2
CLS430290	Corning® 50 mL centrifuge tubes
G5516	Glycerol T5574 Terrific Broth
G3169	GC5™ Competent Cells
P9620	Puromycin Dihydrochloride Ready Made Solution
NA0410	GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit
NA9604	GenElute™ HP 96-Well Plasmid Miniprep Kit
NA1020	GenElute™ PCR Clean-Up Kit RTN350 GenElute™ Mammalian Total RNA Miniprep Kit
T9424	TRI Reagent® RNA Isolation Reagent
D3687	DirectLoad™ PCR 100 bp Low Ladder
D7058	DirectLoad™ Wide Range DNA Marker
D9307	JumpStart™ Taq DNA Polymerase
P0982	JumpStart™ REDTaq® ReadyMix™ Reaction Mix
R2523	REDTaq® ReadyMix™ PCR Reaction Mix
D1806	Taq DNA Polymerase from Thermus aquaticus
CLS4487	Corning® Costar® Stripette® serological pipettes, 5 mL
CLS4489	Corning® Costar® Stripette® serological pipettes, 25 mL
CLS4490	Corning® Costar® Stripette® serological pipettes, 50 mL
HT90132	Crystal violet solution
15205	Blasticidine S hydrochloride
H0654	Hygromycin B solution from Streptomyces hygroscopicus
G1N70	GenElute™ Mammalian Genomic DNA Miniprep Kits
C2874	CryoStor® cell cryopreservation media
H6648	Hanks’ Balanced Salt solution
H9268	Hexadimethrine bromide
D8662	Dulbecco’s Phosphate Buffered Saline with MgCl2 and CaCl2

Volume Price Discounts

Product	Standard Offering	Quantities 25 - 100	Quantities 101-1000
Sanger QuickPick™ KO Bacteria Glycerol Stocks	500 µL or 120 µL of bacteria glycerol stock	20% discount	40% discount
Sanger QuickPick™ KO Plasmid DNA	1 µg Plasmid DNA at 20 ng/µL	20% discount*	40% discount*
Sanger QuickPick™ KO Lentivirus	1 x 200 µL lentivirus, minimum titer 1e6 TU/mL	20% discount**	40% discount**
Custom CRISPR Plasmid DNA	1 µg Plasmid DNA at 20 ng/µL	15% discount*	30% discount*
Custom CRISPR Lentivirus	1 x 200 µL lentivirus, minimum titer 1e6 TU/mL	15% discount**	30% discount**
Custom CRISPR IVT RNA	20 µL IVT RNA at 200 ng/µL	15% discount	30% discount

* Discount does not apply to upcharge for DNA amounts > 1 µg
** Discount does not apply to upcharges for larger volume, higher titer, or additional aliquoting

Vector Maps and Sequences

Click on the images below for a larger view.

Product Guarantee

We are so confident in the performance of our SygRNA^® products, that we fully guarantee the quality and performance of any gRNA we produce, including custom sequences. If your synthetic guides do not yield detectable cleavage at the intended target site, we will provide you a one-time replacement, free of charge.

To qualify for this guarantee, please send an image or sequencing data from a single experiment demonstrating detectable cleavage using one of our positive controls, side-by-side with the negative results from your SygRNA^® guide. To receive your replacement, simply email us and include sample data from a representative experiment (T7E1, TIDE, or NGS).

 

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