CRISPR Essentials: CRISPR gRNAs, Cas9 and Related Products

Compare and contrast the features of a wide variety of guide RNA (gRNA) and Cas9 products for in vitro and in vivo CRISPR experiments. Select the best formats for your mammalian or plant applications: SygRNA® synthetic gRNA, plasmid DNA, lentiviral particles, and proteins.

CRISPR Formats

§ Modified tracrRNA is needed when using modified crRNA.

SygRNA® synthetic CRISPR two-part system requires both crRNA and tracrRNA to complex with Cas9. When ordering sgRNA or crRNA:tracrRNA, be sure to select the species that matches your Cas9 source.

View our protocols and frequently asked questions to learn more about SygRNA® synthetic CRISPR.  


Bacteria Glycerol Stocks: provided in Terrific Broth supplemented with carbenicllin (100 µg/mL) and 10% glycerol

For Sanger QuickPick™ Knockout gRNAs or custom gRNAs

Learn more about Sanger QuickPick Screening Libraries and Clones
 

No. of Clones Ordered Techware Volume Product Availability
1-24 Individually labeled vials 500 µL
 Ships in 2-3 days
25+ 96 well plates 120 µL
 Ships in 3-10 business days


Plasmid DNA: provided in 1xTris-EDTA

For Sanger QuickPick™ Knockout gRNAs or custom gRNAs

Learn more about Sanger QuickPick Screening Libraries and Clones
 

Quantity of DNA ordered Concentration Techware Volume Product Availability– Sanger QuickPick™ Knockout Clones Product Availability– Custom CRISPR Clones
1 µg 20 ng/µL 1-24 clones: individually labeled vials

25+ clones: 96 well plates		 50 µL
 Ships in 3 weeks; additional time for clone quantities > 100 Ships in 4 weeks; additional time for clone quantities > 100
10 µg

25 µg

100 µg		 Concentration will be reported with product individually labeled vials Varies depending on concentration Ships in 3-4 weeks; additional time for clone quantities > 25 Ships in 4-5 weeks; additional time for clone quantities > 25



Lentivirus: provided in Dulbecco's Modified Eagle's Medium supplemented with 10% Fetal Bovine Serum and 1% Penicillin/Streptomycin

For Sanger QuickPick™ Knockout gRNAs or custom gRNAs

Learn more about Sanger QuickPick Screening Libraries and Clones

Web ordered lentivirus clones are provided in individually labeled vials. Requests for aliquots in plates should be submitted to CRISPR@sial.com.

Volume Titer
106 107 108 109
100 µL N/A N/A 2 x 50 µL
4 x 25 µL		 5 x 20 µL
200 µL 1 x 200 µL
2 x 100 µL
4 x 50 µL
8 x 25 µL		 1 x 200 µL
2 x 100 µL
4 x 50 µL
8 x 25 µL		 4 x 50 µL
8 x 25 µL		 10 x 20 µL
1 mL 5 x 200 µL
10 x 100 µL
20 x 50 µL
40 x 25 µL		 5 x 200 µL
10 x 100 µL
20 x 50 µL
40 x 25 µL		 20 x 50 µL
40 x 25 µL		 50 x 20 µL
2 mL 10 x 200 µL
20 x 100 µL
40 x 50 µL
80 x 25 µL		 10 x 200 µL
20 x 100 µL
40 x 50 µL
80 x 25 µL		 40 x 50 µL
80 x 25 µL		 100 x 20 µL
5 mL 25 x 200 µL
50 x 100 µL
100 x 50 µL
200 x 25 µL		 25 x 200 µL
50 x 100 µL
100 x 50 µL
200 x 25 µL		 100 x 50 µL
200 x 25 µL		 N/A



Sanger QuickPick™ Knockout gRNA Lentivirus Manufacturing Timelines

Please allow for additional shipping time
 

Volume Titer
106 107 108 109
100 µL
 N/A N/A 5 weeks 5 weeks
200 µL
 4 weeks 4 weeks 5 weeks 5 weeks
1 mL 5 weeks 5 weeks 5 weeks 5 weeks
2 mL 5 weeks 5 weeks 5 weeks 5 weeks
5 mL 5 weeks 5 weeks 5 weeks N/A



MISSION™ CRISPR gRNA Lentivirus Manufacturing Timelines

Please allow for additional shipping time

Volume Titer
106 107 108 109
100 µL
 N/A N/A 6 weeks 6 weeks
200 µL
 5 weeks 5 weeks 6 weeks 6 weeks
1 mL 6 weeks 6 weeks 6 weeks 6 weeks
2 mL 6 weeks 6 weeks 6 weeks 6 weeks
5 mL 6 weeks 6 weeks 6 weeks N/A



In Vitro Transcribed RNA (IVT): provided in Molecular Grade Water (DNase-, RNase-, Protease-free)
 

Techware Volume & Concentration Product Availability
Individually labeled vials 20 µL, 200 ng/µL Ships in 5 weeks; allow additional time for clone quantities > 25.

Cas9 Products

Click on the product number to order and see additional product details
 

Cas9 Proteins

Product No. Product Description/Application
CAS9GFPPRO
 Wild-type SpCas9-EGFP Fusion – NEW!
 Original Wild-Type Cas9 fused to enhanced green fluorescent protein. Ideal for flow cytometry applications and visualization of transfected RNP complexes. Highly efficient
ECAS9GFPPR
 Enhanced specificity SpCas9-EGFP Fusion – NEW!
 Cas9 variant with Improved specificity fused to enhanced green fluorescent protein. Ideal for flow cytometry applications, fluorescence microscopy and for experiments that require high accuracy
CAS9PROT Wild-type SpCas9 Original , wild-type Cas9; simple and economical; efficient in a broad range of gene editing applications
ESPCAS9PRO Enhanced specificity SpCas9
 Cas9 variant with improved specificity ideal for experiments that require high accuracy
CAS9D10APR Cas9D10A Nickase A two-gRNA system with improved specificity and decreased off-target potential for specialized genome-editing applications
DCAS9PROT dCas9-3XFLAG™ Biotin Catalytically inactive SpCas9 protein containing a 3XFLAGTM tag and Biotin; ideal for DNA detection and isolation and is a component of the proxy-CRISPR method
FNCAS9PROT FnCas9 Protein Ideal for synthetic biology or cell-free applications

 

Cas9 Plasmids and Lentivirus
 

Product No. Description Format Selection
Method
CAS9P SpCas9 plasmid Plasmid DNA  
CAS9GFPP SpCas9 GFP Plasmid Plasmid DNA Fluorophore
(GFP)
CAS9RFPP SpCas9 RFP Plasmid Plasmid DNA Fluorophore
(RFP)
CAS9GEMP SpCas9 GFP Geminin Plasmid Plasmid DNA Fluorophore
(GFP)
CAS9D10AP SpCas9-D10A Nickase Plasmid Plasmid DNA  
CAS9D10AGFPP SpCas9-D10A Nickase GFP Plasmid Plasmid DNA Fluorophore
(GFP)
CAS9D10ARFPP SpCas9-D10A Nickase RFP Plasmid Plasmid DNA Fluorophore
(RFP)
DCAS9P SpdCas9 Plasmid Plasmid DNA  
DCAS9P300 SpdCas9-p300 Activator
Plasmid		 Plasmid DNA Fluorophore
(GFP)
DCAS9P300RFP SpdCas9-p300 RFP Activator
Expression Plasmid		 Plasmid DNA Fluorophore
(RFP)
FNCAS9P FnCas9 Plasmid Plasmid DNA  
CAS9MRNA SpCas9 mRNA mRNA  
CAS9D10AMRNA SpCas9-D10A Nickase mRNA mRNA  
CAS9BST SpCas9 Blasticidin Lenti Plasmid Plasmid DNA Blasticidin
CAS9HYGROVP SpCas9 Hygromycin Lenti Plasmid Plasmid DNA Hygromycin
CAS9NEO SpCas9 Neomycin Lenti Plasmid Plasmid DNA Neomycin
CAS9ZEOVP SpCas9 Zeo Lenti Plasmid Plasmid DNA Zeo
CAS9GFPVP SpCas9 GFP Lenti Plasmid Plasmid DNA Fluorophore
(GFP)
CAS9RFPVP SpCas9 RFP Lenti Plasmid Plasmid DNA Fluorophore
(RFP)
ESPCAS9BST High Fidelity Sp Cas9 Blasticidin Lenti Plasmid Plasmid DNA Blasticidin
LVCAS9BST SpCas9 Blasticidin Lenti Particles Lentivirus Blasticidin
LVCAS9NEO SpCas9 Neomycin Lenti Particles Lentivirus Neomycin
CAS9BAC1P
 SpCas9 Lambda Red Homologous Recombination Plasmid for E.coli
 Plasmid DNA Ampicillin

 

SAM CRISPR Activator Cas9 Products


 

Product No. Description Compatible gRNA vector System Format Selection Method Comment  
SAMHELPERV SAM CRISPRa Helper Construct Kit Lentiviral Transduction Particles LV06 & LV07 Lentiviral Lentivirus Blasticidin & Hygromycin Order compatible
gRNA separately

Kit contains 2
components :

• dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1
• MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
            SAMHELPERP   SAM CRISPRa Helper Construct Kit Plasmid DNA LV06 & LV07 Lentiviral Plasmid DNA Blasticidin & Hygromycin Order compatible gRNA separately

Kit contains 2
components :

• dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1
• MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
SAMVP64BSTV dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 Lentiviral Transduction Particles     LV06 & LV07 Lentiviral Lentivirus Blasticidin Order compatible
gRNA separately
 
Requires MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
SAMVP64BST dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 Plasmid DNA LV06 & LV07 Lentiviral Plasmid DNA Blasticidin Order compatible gRNA separately
 
Requires MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
  SAMMS2HYGV   MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2 Lentiviral Transduction Particles LV06 & LV07 Lentiviral Lentivirus Hygromycin Order compatible gRNA separately
 
Requires dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1
            SAMMS2HYG     MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2 Plasmid DNA LV06 & LV07 Lentiviral Plasmid DNA Hygromycin Order compatible gRNA separately
 
Requires dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1

Vectors


Mammalian System Vectors
 

Vector No. Description Order
Clones		 Cas9/gRNA
Content		 Format
Availability		 Other Features
Fluorophore Cas9 type
p01 U6-gRNA:CMV-Cas9-2A-tGFP p01 clones Cas9+gRNA Plasmid DNA GFP S. pyogenes
p02 U6-gRNA:CMV-Cas9-2A-tRFP p02 clones Cas9+gRNA Plasmid DNA RFP S. pyogenes
p04 U6-gRNA:CMV-eCas9-2a-tGFP p04 clones Cas9+gRNA Plasmid DNA GFP enhanced specificity
p03 U6-gRNA p03 clones gRNA only Plasmid DNA    



Lentiviral Vectors
 

Vector
No.		 Description Order
Clones		 Cas9/gRNA Content Format
Availability		 Selection
Method
LV01 U6-gRNA:ef1a-puro-2A-Cas9-2A-tGFP LV01 clones Cas9+gRNA Plasmid DNA &
Lentivirus		 Fluorophore (GFP) & Puromycin
LV05 U6-gRNA:EF1a-Cas9+FLAG-2A-Puro LV05 clones† Cas9+gRNA Plasmid & Lentivirus Puromycin
LV06 CRISPRa SAM U6-gRNA:EF1a-Puro LV06 clones† CRISPR activator gRNA Plasmid & Lentivirus Puromycin
LV07 CRISPRa SAM U6-gRNA:EF1a-Zeo LV07 clones† CRISPR activator gRNA Plasmid & Lentivirus Zeo
LV04 U6-gRNA:hPGK-puro-2A-tBFP Sanger QP KO clones†
 gRNA only Plasmid DNA &
Lentivirus		 Fluorophore (BFP) & Puromycin
LV03 U6-gRNA:ef1a-tGFP LV03 clones gRNA only Plasmid DNA &
Lentivirus		 Fluorophore (GFP)
LV02 U6-gRNA:ef1a-puro LV02 clones gRNA only Plasmid DNA &
Lentivirus		 Puromycin

†Web ordering is currently available in select countries. Global web ordering capabilities are in development. Please contact CRISPR@sial.com for questions or assistance placing clone orders.



Plant Vectors
 

Vector
No.
 Description Order
Clones		 Cas9/gRNA
Content		 Format
Availability		 Species Plasmid
Type		 Selection
p63 (dicot)U6-gRNA:CMV-Cas9-beta glucuronidase (Agrobacterium plasmid) p63 clones‖ Cas9+
gRNA		 Plasmid
DNA		 Dicot Agro-bacterium Beta
Glucuron-
idase
p55 (dicot)U6-gRNA:CMV-Cas9-bar (Agrobacterium plasmid) p55 clones‖ Cas9+
gRNA		 Plasmid
DNA		 Dicot Agro-bacterium Biala-
phos
p53 (dicot)U6-gRNA:CMV-Cas9-hyg (Agrobacterium plasmid) p53 clones‖ Cas9+
gRNA		 Plasmid
DNA		 Dicot Agro-bacterium Hygro-
mycin
p54 (dicot)U6-gRNA:CMV-Cas9-neo (Agrobacterium plasmid) p54 clones‖ Cas9+
gRNA		 Plasmid
DNA		 Dicot Agro-bacterium Neo-
mycin
p61 (dicot)U6-gRNA:CMV-Cas9-bar (bioballistic plasmid) p61 clones‖ Cas9+
gRNA		 Plasmid
DNA		 Dicot Bio-Ballistic Biala-
phos
p59 (dicot)U6-gRNA:CMV-Cas9-hyg (bioballistic plasmid) p59 clones‖ Cas9+
gRNA		 Plasmid
DNA		 Dicot Bio-Ballistic Hygro-
mycin
p60 (dicot)U6-gRNA:CMV-Cas9-neo (bioballistic plasmid) p60 clones‖ Cas9+
gRNA		 Plasmid
DNA		 Dicot Bio-Ballistic Neo-
mycin
p62 (monocot)U6-gRNA:CMV-Cas9-beta glucuronidase (Agrobacterium plasmid) p62 clones‖ Cas9+
gRNA		 Plasmid
DNA		 Monocot Agro-bacterium Beta
Glucuron-
idase
p52 (monocot)U6-gRNA:CMV-Cas9-bar (Agrobacterium plasmid) p52 clones‖ Cas9+
gRNA		 Plasmid
DNA		 Monocot Agro-bacterium Biala-
phos
p50 (monocot) U6-gRNA:CMV-Cas9-hyg (Agrobacterium plasmid) p50 clones‖ Cas9+
gRNA		 Plasmid
DNA		 Monocot Agro-bacterium Hygro-
mycin
p51 (monocot)U6-gRNA:CMV-Cas9-neo (Agrobacterium plasmid) p51 clones‖ Cas9+
gRNA		 Plasmid
DNA		 Monocot Agro-bacterium Neo-
mycin
p58 (monocot)U6-gRNA:CMV-Cas9-bar (bioballistic plasmid) p58 clones‖ Cas9+
gRNA		 Plasmid
DNA		 Monocot Bio-Ballistic Biala-
phos
p56 (monocot)U6-gRNA:CMV-Cas9-hyg (bioballistic plasmid) p56 clones‖ Cas9+
gRNA		 Plasmid
DNA		 Monocot Bio-Ballistic Hygro-
mycin
p57 (monocot)U6-gRNA:CMV-Cas9-neo (bioballistic plasmid) p57 clones‖ Cas9+
gRNA		 Plasmid
DNA		 Monocot Bio-Ballistic Neo-
mycin

‖ Web ordering is currently available in select countries. For countries where web ordering is not yet available, please fill out the Custom Plant CRISPR Ordering Form

 

Bacterial Editors

Vector No. Description Order Clones Cas9/gRNA Content Format Availability Selection
p10 (bacteria) J23119-gRNA-amp
 p10 clones
 gRNA only
 Plasmid DNA Ampicillin

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CRISPR Controls

Click on the product number to order and see additional product details
 

Product No. Description Vector Control Type System Format gRNA Type Selection Method
CRISPR06 CRISPR Universal Non-target Negative Control 1, Cas9+gRNA Plasmid p01 Neg.
 Mammalian Plasmid
DNA		 Cas9+gRNA Fluorophore (GFP)
CRISPR07 CRISPR Universal Non-target Negative Control 2, Cas9+gRNA Plasmid p01 Neg. Mammalian Plasmid
DNA		 Cas9+gRNA Fluorophore (GFP)
CRISPR08 CRISPR Universal Non-target Negative Control 3, Cas9+gRNA Plasmid p01 Neg. Mammalian Plasmid
DNA		 Cas9+gRNA Fluorophore (GFP)
CRISPR17 CRISPR Activator Human Oct4 Positive Control Plasmid, gRNA Only Expression Plasmid p03 Pos. Mammalian Plasmid
DNA		 gRNA only  
CRISPR12 CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lenti Plasmid (vector) LV01 Neg. Lentiviral Plasmid
DNA		 Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR18 CRISPR-Lenti Universal Non-target Negative Control 1, gRNA Only Lenti Plasmid (Sanger vector) LV04 Neg. Lentiviral Plasmid
DNA		 gRNA only Fluorophore (BFP) & Puromycin
CRISPR19 CRISPR-Lenti Universal Non-target Negative Control 2, gRNA Only Lenti Plasmid (Sanger vector) LV04 Neg. Lentiviral Plasmid
DNA		 gRNA only Fluorophore (BFP) & Puromycin
CRISPR20 CRISPR-Lenti Universal Non-target Negative Control 3, gRNA Only Lenti Plasmid (Sanger vector) LV04 Neg. Lentiviral Plasmid
DNA		 gRNA only Fluorophore (BFP) & Puromycin
CRISPR12V CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lentiviral Particles (vector) LV01 Neg. Lentiviral Lentivirus Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR12H CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lentiviral Particles, High Titer (vector) LV01 Neg. Lentiviral Lentivirus Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR15H Gecko2-Lenti Universal Non-Target Negative Control 1, gRNA Only High Titer Lentiviral Particles (Gecko2 vector) LV02 Neg. Lentiviral Lentivirus gRNA only Puromycin
CRISPR18V CRISPR-Lenti Universal Non-target Negative Control 1, gRNA Only Lentiviral Particles (Sanger vector) LV04 Neg. Lentiviral Lentivirus gRNA only Fluorophore (BFP) & Puromycin
CRISPR19V CRISPR-Lenti Universal Non-target Negative Control 2, gRNA Only Lentiviral Particles (Sanger vector) LV04 Neg. Lentiviral Lentivirus gRNA only Fluorophore (BFP) & Puromycin
CRISPR20V CRISPR-Lenti Universal Non-target Negative Control 3, gRNA Only Lentiviral Particles (Sanger vector) LV04 Neg. Lentiviral Lentivirus gRNA only Fluorophore (BFP) & Puromycin
CRISPR01 CRISPR Human EMX1 Positive Control Plasmid, gRNA Only Expression Plasmid, includes Cas9 Expression Plasmid (vector) p03 Pos. Mammalian Plasmid
DNA		 gRNA only  
CRISPR02 CRISPR Nickase Human EMX1 Positive
Control Plasmids (gRNA Only Expression
Plasmids, Includes Cas9-D10A Plasmid)		 p03 Pos. Mammalian Plasmid
DNA		 gRNA only  
CRISPR11 CRISPR-Lenti Human EMX1 Positive Control, Cas9+gRNA Lenti Plasmid (vector) LV01 Pos. Lentiviral Plasmid
DNA		 Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR13 CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lenti Plasmid (vector) LV01 Pos. Lentiviral Plasmid
DNA		 Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR21 CRISPR-Lenti Human HPRT1 Positive Control, gRNA Only Lenti Plasmid (Sanger vector) LV04 Pos. Lentiviral Plasmid
DNA		 gRNA only Fluorophore (BFP) & Puromycin
CRISPR11V CRISPR-Lenti Human EMX1 Positive Control, Cas9+gRNA Lentiviral Particles (vector) LV01 Pos. Lentiviral Lentivirus Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR13V CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lentiviral Particles (vector) LV01 Pos. Lentiviral Lentivirus Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR13H CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lentiviral Particles, High Titer (vector) LV01 Pos. Lentiviral Lentivirus Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR16H Gecko2-Lenti Human HPRT1 Positive Control, gRNA Only High Titer Lentiviral Particles (Gecko2 vector) LV02 Pos. Lentiviral Lentivirus gRNA only Puromycin
CRISPR21V CRISPR-Lenti Human HPRT1 Positive Control, gRNA Only Lentiviral Particles (Sanger vector) LV04 Pos. Lentiviral Lentivirus gRNA only Fluorophore (BFP) + Puromycin
CRISPRPL01 CRISPR GUS GAPDH Reporter Control for Monocots p62 Pos. Plant Plasmid
DNA		 Cas9+gRNA Beta
Glucuronidase
CRISPRPL02 CRISPR GUS GAPDH Reporter Control for Dicots p63 Pos. Plant Plasmid
DNA		 Cas9+gRNA Beta
Glucuronidase

CRISPR Companion Products

 

HSOLIGOINT CRISPR Integration Kit - Use to determine baseline for Homology Directed Repair for cell lines
SHP002 CRISPR & MISSION® Lentiviral Packaging Mix
T1706 T1706 TransIT-CRISPR® Transfection Reagent
D6171 Dulbecco’s Modified Eagle’s Medium - high glucose, HEPES modification
D8537 Dulbecco’s Phosphate Buffered Saline
D5796 Dulbecco’s Modified Eagle’s Medium - high glucose P4333 Penicillin-Streptomycin
T3924 Trypsin-EDTA solution
G7513 L-Glutamine solution
CLS430720 Corning® cell culture flasks, 75 cm2
CLS430825 Corning® cell culture flasks, 150 cm2
CLS431082 Corning® cell culture flask, 225 cm2
CLS430290 Corning® 50 mL centrifuge tubes
G5516 Glycerol T5574 Terrific Broth
G3169 GC5™ Competent Cells
P9620 Puromycin Dihydrochloride Ready Made Solution
NA0410 GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit
NA9604 GenElute™ HP 96-Well Plasmid Miniprep Kit
NA1020 GenElute™ PCR Clean-Up Kit RTN350 GenElute™ Mammalian Total RNA Miniprep Kit
T9424 TRI Reagent® RNA Isolation Reagent 
D3687 DirectLoad™ PCR 100 bp Low Ladder
D7058 DirectLoad™ Wide Range DNA Marker
D9307 JumpStart™ Taq DNA Polymerase
P0982 JumpStart™ REDTaq® ReadyMix™ Reaction Mix
R2523 REDTaq® ReadyMix™ PCR Reaction Mix
D1806 Taq DNA Polymerase from Thermus aquaticus
CLS4487 Corning® Costar® Stripette® serological pipettes, 5 mL
CLS4489 Corning® Costar® Stripette® serological pipettes, 25 mL
CLS4490 Corning® Costar® Stripette® serological pipettes, 50 mL 
HT90132 Crystal violet solution
15205 Blasticidine S hydrochloride
H0654 Hygromycin B solution from Streptomyces hygroscopicus
G1N70 GenElute™ Mammalian Genomic DNA Miniprep Kits
C2874 CryoStor® cell cryopreservation media
H6648 Hanks’ Balanced Salt solution
H9268 Hexadimethrine bromide
D8662 Dulbecco’s Phosphate Buffered Saline with MgCl2 and CaCl2

Volume Price Discounts

Product Standard Offering Quantities
25 - 100		 Quantities 101-1000
Sanger QuickPick™ KO Bacteria Glycerol Stocks 500 µL or 120 µL of bacteria glycerol stock 20% discount 40% discount
Sanger QuickPick™ KO Plasmid DNA 1 µg Plasmid DNA at 20 ng/µL 20% discount* 40% discount*
Sanger QuickPick™ KO Lentivirus 1 x 200 µL lentivirus, minimum titer 1e6 TU/mL 20% discount** 40% discount**
Custom CRISPR Plasmid DNA 1 µg Plasmid DNA at 20 ng/µL 15% discount* 30% discount*
Custom CRISPR Lentivirus 1 x 200 µL lentivirus, minimum titer 1e6 TU/mL 15% discount** 30% discount**
Custom CRISPR IVT RNA 20 µL IVT RNA at 200 ng/µL 15% discount 30% discount

* Discount does not apply to upcharge for DNA amounts > 1 µg
** Discount does not apply to upcharges for larger volume, higher titer, or additional aliquoting

Vector Maps and Sequences

Click on the images below for a larger view.

p01 Vector

Download Fully Annotated p01 Map
Download p01 FASTA Sequence

 

p02 Vector

Download Fully Annotated p02 Map
Download p02 FASTA Sequence

 

p03 Vector

Download Fully Annotated p03 Map
Download p03 FASTA Sequence

 

p04 Vector

Download Fully Annotated p04 Map
Download p04 FASTA Sequence

 

p05 Vector

Download Fully Annotated p05 Map
Download p05 FASTA Sequence

 

p10 Vector

Download Fully Annotated p10 Map
Download p10 FASTA Sequence

 

p50 Vector

Download Fully Annotated p50 Map
Download p50 FASTA Sequence

 

p51 Vector

Download Fully Annotated p51 Map
Download p51 FASTA Sequence

 

p52 Vector

Download Fully Annotated p52 Map
Download p52 FASTA Sequence

 

p53 Vector

Download Fully Annotated p53 Map
Download p53 FASTA Sequence

 

p54 Vector

Download Fully Annotated p54 Map
Download p54 FASTA Sequence

 

p55 Vector

Download Fully Annotated p55 Map
Download p55 FASTA Sequence

 

p56 Vector

Download Fully Annotated p56 Map
Download p56 FASTA Sequence

 

p57 Vector

Download Fully Annotated p57 Map
Download p57 FASTA Sequence

 

p58 Vector

Download Fully Annotated p58 Map
Download p58 FASTA Sequence

 

p59 Vector

Download Fully Annotated p59 Map
Download p59 FASTA Sequence

 

p60 Vector

Download Fully Annotated p60 Map
Download p60 FASTA Sequence

 

p61 Vector

Download Fully Annotated p61 Map
Download p61 FASTA Sequence

 

p62 Vector

Download Fully Annotated p62 Map
Download p62 FASTA Sequence

 

p63 Vector

Download Fully Annotated p63 Map
Download p63 FASTA Sequence

 

LV01 Vector

Download Fully Annotated LV01 Map
Download LV01 FASTA Sequence

 

LV02 Vector

Download Fully Annotated LV02 Map
Download LV02 FASTA Sequence

 

LV03 Vector

Download Fully Annotated LV03 Map
Download LV03 FASTA Sequence

 

LV04 Vector

Download Fully Annotated LV04 Map
Download LV04 FASTA Sequence

 

LV05 Vector

Download Fully Annotated LV05 Map
Download LV05 FASTA Sequence

 

LV06 Vector

Download Fully Annotated LV06 Map
Download LV06 FASTA Sequence

 

LV07 Vector

Download Fully Annotated LV07 Map
Download LV07 FASTA Sequence

 

Product Guarantee

We are so confident in the performance of our SygRNA® products, that we fully guarantee the quality and performance of any gRNA we produce, including custom sequences. If your synthetic guides do not yield detectable cleavage at the intended target site, we will provide you a one-time replacement, free of charge.

To qualify for this guarantee, please send an image or sequencing data from a single experiment demonstrating detectable cleavage using one of our positive controls, side-by-side with the negative results from your SygRNA® guide. To receive your replacement, simply email oligotechserv@merckgroup.com and include sample data from a representative experiment (T7E1, TIDE, or NGS).

 

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