Extraction, isolation, purification, separation, detection—we know your time is better spent analyzing data than performing arduous protocols before you can even measure your proteins of interest. We can help with the most efficient approaches to cloning and expression with a wide variety of cells, reagents, and vectors. Our membrane engineers have developed devices expressly for desalting, concentrating, and buffer exchange that save your proteins, and save you time and protocol steps. A comprehensive offering of protein preparation products provide gentle extraction, high-yield isolation and purification, and application-verified detection tools for techniques like electrophoresis and Western blot, ELISA, multiplex, and mass spectrometry.
| Protein Expression |
| Find the best system for recombinant expression. • Competent bacteria • Yeast expression systems • Vectors • Competent cells • Transformation/transfection reagents |
| Lysis & Protein Extraction |
| Detergents and protease inhibitors to maximize cell lysis efficiency and obtain the high protein yield. • Busters • Benzonase • Inhibitor cocktails • Roche cOmplete™ protease inhibitors |
| Protein Purification & Isolation |
| Agarose resins & magnetic beads for diverse protein purification strategies. • Antibody Purification • Recombinant Protein Purification • Ion Exchange Purification • Biotinylated Molecule Purification • Protein Immobilization |
| Protein Concentration & Buffer Exchange |
| Fast and gentle sample concentration, buffer exchange, desalting and dialysis. • Amicon® Ultra centrifugal filters • Large volume concentration • D-tube dialyzers |
| Separation/Gel Electrophoresis |
| Precast gels, buffers & solvents, and prestained molecular weight markers to improve protein resolution with SDS-PAGE. |
| Western Blotting |
| Get the right band when you use tools optimized for your target protein. • Transfer membranes • Blocking buffers • Detection reagents • Western blotting protocol |
| Antibodies |
| Over 90,000 world-class primary and secondary antibodies, conjugates and technical resources. |
| Protein Assays & Analysis |
| Accurately interrogate the proteins relevant to your system. • Duolink® PLA protein detection technology • ELISA • MILLIPLEX® multiplex assays using Luminex® technology • SMC (single molecule counting) assay |
| Proteomics & Mass Spectrometry |
| The expanding field of proteomics requires high-quality mass spec reagents. • MS internal standards • Seppro Protein Depletion • Proteomics Core • Glycoprotein Analysis |
| Protein Quantitation |
| Trusted methods for quantitation including BCA, Bradford and Lowry. • Tools for Protein Quantitation |
| Core Protein Reagents |
| Essential reagents and tools to support your protein research. • Detergents • Enzymes and Proteins • Membrane protein reagents • General protease inhibitors • Reagents for protein stabilization |
| Life Science Labware & Equipment |
| From mixing and storage to separation and transfer, we offer: • Tissue homogenizers • Electrophoresis supplies • Glassware and Labware • Centrifugation supplies • Filtration supplies |