Custom DNA and RNA Oligos & qPCR Probes Learning Center

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SARS-CoV-2 Coronavirus Detection – Target detection and positive control qPCR assays

OligoEvaluator™ Calculator

The OligoEvaluator™ oligonucleotide sequence calculator provides oligo analysis values.

DNA Oligonucleotide Synthesis

Phosphoramidite chemistry, developed in the 1980s and later enhanced with solid-phase supports and automation, is the method of choice for DNA oligonucleotide manufacturing.

Protocol for Annealing Oligonucleotides

The annealing process is divided into two main steps: 1) dissolution, and 2) annealing, which are performed by either heat block or thermocycler. Although each oligonucleotide is provided in a specific amount, for best results, measure the OD before use.

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