LOCUS p57 9739 bp DNA circular 22-AUG-2017 DEFINITION . ACCESSION urn.local...9t-7o8q9kz KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers primer 63..86 /label="Sequencing XhoI Rev primer" misc_feature 179..973 /label="Kan R" primer_bind 426..445 /label="TDNA-pUCRev" misc_feature 1273..1946 /label="pUC Ori" primer_bind 1823..1842 /label="pUC-THMS2 primer Forw" misc_feature 1981..1986 /Recognition_pattern="G^TCGAC" /label="SalI" /note="Geneious type: restriction site" misc_feature 2005..2010 /Recognition_pattern="T^CTAGA" /label="XbaI" /note="Geneious type: restriction site" misc_feature 2011..2036 /label="T-DNA LB" misc_feature 2037..2044 /Recognition_pattern="GTTT^AAAC" /label="PmeI" /note="Geneious type: restriction site" loci complement(2045..3160) /label="NPTII" misc_feature 3161..3166 /Recognition_pattern="G^GTACC" /label="Acc65I" /note="Geneious type: restriction site" misc_feature complement(3167..3997) /label="35 S promoter" misc_feature 3998..4003 /Recognition_pattern="G^AATTC" /label="EcoRI" /note="Geneious type: restriction site" allele 4004..4366 /label="Wh-U6" misc_feature 4251..4256 /Recognition_pattern="G^TCGAC" /label="SalI" /note="Geneious type: restriction site" attenuator 4371..4377 /label="re-AarI" attenuator 4386..4392 /label="AarI" attenuator 4397..4479 /label="Mo-sgRNA" misc_feature 4480..4485 /label="Barcode" misc_feature 4486..4491 /Recognition_pattern="A^AGCTT" /label="HindIII" /note="Geneious type: restriction site" -35_signal 4520..5300 /label="35S promoter" misc_feature 5334..5339 /Recognition_pattern="G^CTAGC" /label="NheI" /note="Geneious type: restriction site" C_region 5340..9471 /label="ZmCas9" misc_feature 7528..7533 /Recognition_pattern="G^GATCC" /label="BamHI" /note="Geneious type: restriction site" misc_feature 9472..9477 /Recognition_pattern="C^CCGGG" /label="XmaI" /note="Geneious type: restriction site" misc_feature 9661..9666 /Recognition_pattern="AGG^CCT" /label="StuI" /note="Geneious type: restriction site" misc_feature 9667..9690 /label="T-DNA RB" misc_feature 9734..9739 /label="XhoI" ORIGIN 1 ctcgagcttc tactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc 61 gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct cgccgccaag 121 gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat cgtttcgcat 181 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 241 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 301 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 361 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 421 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 481 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 541 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 601 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 661 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg 721 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 781 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 841 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 901 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 961 cgagttcttc tgaattatta acgcttacaa tttcctgatg cggtattttc tccttacgca 1021 tctgtgcggt atttcacacc gcatacaggt ggcacttttc ggggaaatgt gcgcggaacc 1081 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 1141 tgataaatgc ttcaataata gcacgtgcta aaacttcatt tttaatttaa aaggatctag 1201 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 1261 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 1321 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 1381 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 1441 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 1501 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 1561 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 1621 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 1681 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 1741 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 1801 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 1861 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 1921 ggcttttgct ggccttttgc tcacatgttc ttgactcttc gcgatgtacg ggccagatat 1981 gtcgacgtaa aacgacggcc agtgtctaga tggcaggata tattgtggtg taaacagttt 2041 aaacagtgtt ttactcctca tattaacttc ggtcattaga ggccacgatt tgacacattt 2101 ttactcaaaa caaaatgttt gcatatctct tataatttca aattcaacac acaacaaata 2161 agagaaaaaa caaataatat taatttgaga atgaacaaaa ggaccatatc attcattaac 2221 tcttctccat ccatttccat ttcacagttc gatagcgaaa accgaataaa aaacacagta 2281 aattacaagc acaacaaatg gtacaagaaa aacagttttc ccaatgccat aatactcgaa 2341 cgtccggagt tatcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc 2401 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc 2461 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc 2521 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc 2581 gccatgggtc acgacgagat cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag 2641 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc 2701 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt 2761 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc 2821 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc 2881 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag 2941 ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt 3001 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc 3061 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc 3121 tgcgtgcaat ccatcttgtt caatccaagc tcccattgtt ggtacccagc ttgggtctag 3181 tcgtattaag agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat 3241 gaaatgaact tccttatata gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc 3301 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc 3361 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga 3421 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc 3481 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt 3541 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc 3601 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttatcac atcaatccac 3661 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg 3721 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca 3781 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat 3841 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat 3901 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg 3961 ctccaccatt acataggccc atcggagcta acgcagtgaa ttcgaccaag cccgttattc 4021 tgacagttct ggtgctcaac acatttatat ttatcaagga gcacattgtt actcactgct 4081 aggagggaat cgaactagga atattgatca gaggaactac gagagagctg aagataactg 4141 ccctctagct ctcactgatc tgggtcgcat agtgagatgc agcccacgtg agttcagcaa 4201 cggtctagcg ctgggctttt aggcccgcat gatcgggctt ttgtcgggtg gtcgacgtgt 4261 tcacgattgg ggagagcaac gcagcagttc ctcttagttt agtcccacct cgcctgtcca 4321 gcagagttct gaccggttta taaactcgct tgctgcatca gacttggctc gcaggtggaa 4381 gaatgcacct gcgagagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt 4441 atcaacttga aaaagtggca ccgagtcggt gctttttttg tggccaagct tactgcgtta 4501 gctccgatgg gcctatgtaa tggtggagca cgacactctc gtctactcca agaatatcaa 4561 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg 4621 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa 4681 ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc 4741 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga 4801 agacgttcca accacgtctt caaagcaagt ggattgatgt gataacatgg tggagcacga 4861 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga 4921 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg 4981 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga 5041 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc 5101 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga 5161 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga 5221 ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctgaaa tcaccagtct 5281 ctctctacaa atctatctct taatacgact cactataggg agacccaagc tgggctagca 5341 acaatggaca agaagtacag catcggcctg gacatcggca ccaactctgt tggctgggct 5401 gtgatcaccg acgagtacaa ggtgccgagc aagaagttca aggtcctggg caacaccgac 5461 aggcacagca tcaagaagaa cctgatcggc gccctgctgt tcgactctgg tgagactgct 5521 gaggccacca ggctgaagag gactgctaga agaaggtaca ccaggcgcaa gaacaggatc 5581 tgctacctgc aagagatttt cagcaacgag atggccaagg tggacgacag cttcttccac 5641 aggcttgagg agagcttcct ggtcgaggag gataagaagc acgagaggca cccgatcttc 5701 ggcaacatcg tggatgaggt ggcctaccac gagaagtacc cgaccatcta ccacctccgc 5761 aagaagctgg ttgacagcac cgacaaggct gacctgaggc tgatctacct ggccctggcc 5821 cacatgatca agttcagggg ccacttcctc atcgagggcg acctgaaccc agacaacagc 5881 gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt cgaggagaac 5941 ccgatcaacg cctctggcgt ggacgctaag gctatcctgt ctgccaggct gagcaagagc 6001 aggcgccttg agaaccttat cgctcagctg ccgggcgaga agaagaacgg ccttttcggc 6061 aacctgattg ccctgtctct gggcctgacc ccgaacttca agagcaactt cgacctggcc 6121 gaggacgcca agctccagct gtctaaggac acctacgacg acgacctgga caacctgctt 6181 gctcagatcg gcgaccagta cgccgacctg ttcctggctg ctaagaacct gtctgacgcc 6241 atcctgctga gcgacatcct gagggtgaac accgagatca ccaaggctcc actgagcgcc 6301 tctatgatca agcgctacga cgagcaccac caggacctga ctctgctgaa ggctctggtt 6361 aggcagcagc tgccagagaa gtacaaggag attttcttcg accagagcaa gaacggctac 6421 gccggctaca ttgatggcgg cgcttctcaa gaggagttct acaagttcat caagccgatc 6481 cttgagaaga tggacggcac cgaggagctg ctggttaagc tgaacagaga ggacctgctg 6541 aggaagcaga ggaccttcga caacggcagc atcccgcacc agatccatct gggcgagctg 6601 catgctatcc tgaggcgcca agaggacttc tacccgttcc tgaaggacaa ccgcgagaag 6661 attgagaaga tcctgacctt ccgcatcccg tactacgttg gcccacttgc taggggcaac 6721 agcaggttcg cttggatgac ccgcaagagc gaggagacta tcaccccctg gaacttcgag 6781 gaggtggtgg acaagggcgc ttctgcccag tctttcatcg agaggatgac caacttcgat 6841 aagaacctcc cgaacgagaa ggtgctgccg aagcactctc tgctgtacga gtacttcacc 6901 gtgtacaacg agctgacgaa ggtcaagtac gtgaccgagg gtatgaggaa gccagccttc 6961 ctgagcggcg agcaaaagaa ggccatcgtc gatctgctgt tcaagaccaa ccgcaaggtg 7021 accgtgaagc agctcaagga ggactacttc aagaagatcg agtgcttcga ctccgtcgag 7081 atcagcggcg ttgaggacag gttcaacgct tccctgggca cctaccatga cctgctcaag 7141 attatcaagg acaaggactt cctcgacaac gaggagaatg aggacattct tgaggacatc 7201 gtgctgaccc tgaccctctt cgaggaccgc gagatgatcg aggagaggct caagacctac 7261 gcccacctct tcgacgacaa ggtgatgaag caactgaaga ggcgcaggta cactggctgg 7321 ggcaggcttt ctaggaagct gatcaacggc atcagggaca agcagagcgg caagacgatc 7381 ctcgacttcc tgaagtctga cggcttcgct aaccgcaact tcatgcagct gatccacgac 7441 gacagcctca ccttcaagga ggatatccag aaggcccagg ttagcggcca gggcgattct 7501 ctgcatgagc atatcgctaa cctcgccgga tccccggcta tcaagaaggg catcctgcag 7561 accgtcaaga tcgtggacga gctggtgaag gtgatgggcc acaagccaga gaacatcgtg 7621 atcgagatgg cgcgcgagaa ccagaccact cagaagggcc aaaagaacag ccgcgagcgc 7681 atgaagagga ttgaggaggg cattaaggag ctgggcagcc agatcctcaa ggagcaccca 7741 gttgagaaca cccagctgca gaatgagaag ctgtacctgt actacctgca gaacggcagg 7801 gacatgtacg tggaccaaga gctggacatc aacaggctgt ccgactacga cgttgaccac 7861 atcgtgccgc agagcttcat caaggacgac tccatcgaca acaaggtcct cacccgcagc 7921 gacaagaacc gcggcaagtc tgacaacgtg ccaagcgagg aggtcgtgaa gaagatgaag 7981 aactactggc ggcagctcct gaacgcgaag ctcatcaccc agcgcaagtt cgacaacctg 8041 accaaggctg agagaggcgg cctgagcgag cttgataagg ccggcttcat taagaggcag 8101 ctggtcgaga ctaggcagat cacgaagcac gtggcccaga tcctggacag ccgcatgaac 8161 accaagtacg acgagaacga caagctgatc cgcgaggtga aggttatcac cctgaagtcc 8221 aagctggtga gcgacttccg caaggatttc caattctaca aggtccgcga gatcaacaac 8281 taccaccacg cccacgacgc ctacctgaac gctgttgttg gcaccgccct gatcaagaag 8341 tacccaaagt tggagagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcgcaag 8401 atgatcgcca agtcggagca agagatcggc aaggcgaccg ccaagtactt cttctacagc 8461 aacatcatga atttcttcaa gactgagatc accctggcca acggcgagat cagaaagagg 8521 ccgctcattg agactaatgg cgagactggc gagatcgtgt gggataaggg cagggacttc 8581 gccactgtgc gcaaggtgct gtctatgcca caggtgaaca tcgttaagaa gaccgaggtc 8641 cagaccggcg gcttcagcaa ggagtctatc ctgccgaaga ggaacagcga taagctgatc 8701 gcccggaaga aggactggga cccaaagaag tacggcgggt tcgatagccc aaccgtggct 8761 tactctgtgc tggtggtggc gaaggtcgag aagggcaagt ccaagaagct caagagcgtc 8821 aaggagcttc tgggcatcac catcatggag aggtccagct tcgagaagaa tccaatcgat 8881 ttccttgagg ccaagggcta caaggaggtc aagaaggacc tcatcatcaa gctgccaaag 8941 tactccctgt tcgagttgga gaacggccgc aagaggatgc tggcttctgc tggcgagctt 9001 caaaagggca acgagcttgc cctgccgtcc aagtacgtca acttcctgta cctggccagc 9061 cactacgaga agctgaaggg ctctccagag gacaatgagc aaaagcagct tttcgtcgag 9121 cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttcag caagagggtg 9181 atcctggccg acgccaacct ggataaggtg ctcagcgcct acaacaagca ccgcgacaag 9241 ccaattcgcg agcaggccga gaacatcatc cacctgttca cgctgaccaa cctgggtgcc 9301 ccagcagcct tcaagtactt cgataccacc atcgaccgca agcggtacac ctccactaag 9361 gaggttctgg acgccaccct gatccaccag tctatcactg gcctgtacga gacaaggatc 9421 gacctctcac agctgggcgg cgaccctaag aagaagagga aggtttgatg acccgggtct 9481 ccataataat gtgtgagtag ttcccagata agggaattag ggttcctata gggtttcgct 9541 catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc 9601 aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc ccccgaatta 9661 aggccttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa 9721 cggatattta aaactcgag //