LOCUS p56 9924 bp DNA circular 22-AUG-2017 DEFINITION . ACCESSION urn.local...9o-7o8q998 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers primer 63..86 /label="Sequencing XhoI Rev primer" misc_feature 179..973 /label="Kan R" primer_bind 426..445 /label="pUC-THMS2 primer Rev" misc_feature 1273..1946 /label="pUC Ori" primer_bind 1823..1842 /label="pUC-THMS2 primer Forw" misc_feature 1981..1986 /Recognition_pattern="G^TCGAC" /label="SalI" /note="Geneious type: restriction site" misc_feature 2005..2010 /Recognition_pattern="T^CTAGA" /label="XbaI" /note="Geneious type: restriction site" misc_feature 2011..2036 /label="T-DNA LB" misc_feature complement(2037..2312) /label="NOS terminator" misc_feature complement(2313..3345) /label="Hygromycin R" primer_bind 2373..2392 /label="pUC-THMS2 primer Rev" misc_feature complement(3352..4182) /label="35 S promoter" misc_feature 4183..4188 /Recognition_pattern="G^AATTC" /label="EcoRI" /note="Geneious type: restriction site" allele 4189..4551 /label="Wh-U6" primer 4189..4209 /label="WU6F1" misc_feature 4436..4441 /Recognition_pattern="G^TCGAC" /label="SalI" /note="Geneious type: restriction site" primer 4533..4552 /label="WU6R1" attenuator 4556..4562 /label="re-AarI" attenuator 4571..4577 /label="AarI" attenuator 4582..4664 /label="Mo-sgRNA" misc_feature 4665..4670 /label="Barcode" misc_feature 4671..4676 /Recognition_pattern="A^AGCTT" /label="HindIII" /note="Geneious type: restriction site" -35_signal 4705..5485 /label="35S promoter" misc_feature 5519..5524 /Recognition_pattern="G^CTAGC" /label="NheI" /note="Geneious type: restriction site" C_region 5525..9656 /label="ZmCas9" misc_feature 7713..7718 /Recognition_pattern="G^GATCC" /label="BamHI" /note="Geneious type: restriction site" misc_feature 9657..9662 /Recognition_pattern="C^CCGGG" /label="XmaI" /note="Geneious type: restriction site" misc_feature 9846..9851 /Recognition_pattern="AGG^CCT" /label="StuI" /note="Geneious type: restriction site" misc_feature 9852..9875 /label="T-DNA RB" misc_feature 9919..9924 /label="XhoI" ORIGIN 1 ctcgagcttc tactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc 61 gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct cgccgccaag 121 gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat cgtttcgcat 181 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 241 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 301 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 361 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 421 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 481 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 541 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 601 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 661 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg 721 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 781 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 841 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 901 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 961 cgagttcttc tgaattatta acgcttacaa tttcctgatg cggtattttc tccttacgca 1021 tctgtgcggt atttcacacc gcatacaggt ggcacttttc ggggaaatgt gcgcggaacc 1081 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 1141 tgataaatgc ttcaataata gcacgtgcta aaacttcatt tttaatttaa aaggatctag 1201 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 1261 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 1321 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 1381 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 1441 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 1501 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 1561 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 1621 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 1681 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 1741 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 1801 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 1861 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 1921 ggcttttgct ggccttttgc tcacatgttc ttgactcttc gcgatgtacg ggccagatat 1981 gtcgacgtaa aacgacggcc agtgtctaga tggcaggata tattgtggtg taaacagttt 2041 aaacccgatc tagtaacata gatagcaccg cgcgcgataa tttatcctag tttgcgcgct 2101 atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc 2161 atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca 2221 gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt 2281 gccaaatgtt tgaacgatcg gggagtttaa acttactatt cctttgccct cggacgagtg 2341 ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc 2401 gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg 2461 tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag 2521 agttggtcaa gaccaatgcg gagcatatac gcccggagcc gcggcgatcc tgcaagctcc 2581 ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag 2641 aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg 2701 accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg 2761 aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg 2821 acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca 2881 atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg 2941 ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatggc ctccgcgacc 3001 ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca 3061 cggcgggaga tgcaataggt caggctctcg ctgaactccc caatgtcaag cacttccgga 3121 atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg 3181 gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga 3241 gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga 3301 aacttctcga cagacgtcgc ggtgagttca ggctttttca ttgttggtac ccagcttggg 3361 tctagtcgta ttaagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc 3421 caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg ggattgtgcg 3481 tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga 3541 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg 3601 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca 3661 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg 3721 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact 3781 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa 3841 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt 3901 gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta 3961 tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga 4021 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc 4081 tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg 4141 tcgtgctcca ccattacata ggcccatcgg agctaacgca gtgaattcga ccaagcccgt 4201 tattctgaca gttctggtgc tcaacacatt tatatttatc aaggagcaca ttgttactca 4261 ctgctaggag ggaatcgaac taggaatatt gatcagagga actacgagag agctgaagat 4321 aactgccctc tagctctcac tgatctgggt cgcatagtga gatgcagccc acgtgagttc 4381 agcaacggtc tagcgctggg cttttaggcc cgcatgatcg ggcttttgtc gggtggtcga 4441 cgtgttcacg attggggaga gcaacgcagc agttcctctt agtttagtcc cacctcgcct 4501 gtccagcaga gttctgaccg gtttataaac tcgcttgctg catcagactt ggctcgcagg 4561 tggaagaatg cacctgcgag agttttagag ctagaaatag caagttaaaa taaggctagt 4621 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttgtccgc aagcttactg 4681 cgttagctcc gatgggccta tgtaatggtg gagcacgaca ctctcgtcta ctccaagaat 4741 atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata 4801 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta 4861 gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa 4921 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 4981 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgataa catggtggag 5041 cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct 5101 attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct 5161 atctgtcact tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat 5221 tgcgataaag gaaaggctat cgttcaagat gcctctgccg acagtggtcc caaagatgga 5281 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa 5341 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg 5401 caagaccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc tgaaatcacc 5461 agtctctctc tacaaatcta tctcttaata cgactcacta tagggagacc caagctgggc 5521 tagcaacaat ggacaagaag tacagcatcg gcctggacat cggcaccaac tctgttggct 5581 gggctgtgat caccgacgag tacaaggtgc cgagcaagaa gttcaaggtc ctgggcaaca 5641 ccgacaggca cagcatcaag aagaacctga tcggcgccct gctgttcgac tctggtgaga 5701 ctgctgaggc caccaggctg aagaggactg ctagaagaag gtacaccagg cgcaagaaca 5761 ggatctgcta cctgcaagag attttcagca acgagatggc caaggtggac gacagcttct 5821 tccacaggct tgaggagagc ttcctggtcg aggaggataa gaagcacgag aggcacccga 5881 tcttcggcaa catcgtggat gaggtggcct accacgagaa gtacccgacc atctaccacc 5941 tccgcaagaa gctggttgac agcaccgaca aggctgacct gaggctgatc tacctggccc 6001 tggcccacat gatcaagttc aggggccact tcctcatcga gggcgacctg aacccagaca 6061 acagcgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg 6121 agaacccgat caacgcctct ggcgtggacg ctaaggctat cctgtctgcc aggctgagca 6181 agagcaggcg ccttgagaac cttatcgctc agctgccggg cgagaagaag aacggccttt 6241 tcggcaacct gattgccctg tctctgggcc tgaccccgaa cttcaagagc aacttcgacc 6301 tggccgagga cgccaagctc cagctgtcta aggacaccta cgacgacgac ctggacaacc 6361 tgcttgctca gatcggcgac cagtacgccg acctgttcct ggctgctaag aacctgtctg 6421 acgccatcct gctgagcgac atcctgaggg tgaacaccga gatcaccaag gctccactga 6481 gcgcctctat gatcaagcgc tacgacgagc accaccagga cctgactctg ctgaaggctc 6541 tggttaggca gcagctgcca gagaagtaca aggagatttt cttcgaccag agcaagaacg 6601 gctacgccgg ctacattgat ggcggcgctt ctcaagagga gttctacaag ttcatcaagc 6661 cgatccttga gaagatggac ggcaccgagg agctgctggt taagctgaac agagaggacc 6721 tgctgaggaa gcagaggacc ttcgacaacg gcagcatccc gcaccagatc catctgggcg 6781 agctgcatgc tatcctgagg cgccaagagg acttctaccc gttcctgaag gacaaccgcg 6841 agaagattga gaagatcctg accttccgca tcccgtacta cgttggccca cttgctaggg 6901 gcaacagcag gttcgcttgg atgacccgca agagcgagga gactatcacc ccctggaact 6961 tcgaggaggt ggtggacaag ggcgcttctg cccagtcttt catcgagagg atgaccaact 7021 tcgataagaa cctcccgaac gagaaggtgc tgccgaagca ctctctgctg tacgagtact 7081 tcaccgtgta caacgagctg acgaaggtca agtacgtgac cgagggtatg aggaagccag 7141 ccttcctgag cggcgagcaa aagaaggcca tcgtcgatct gctgttcaag accaaccgca 7201 aggtgaccgt gaagcagctc aaggaggact acttcaagaa gatcgagtgc ttcgactccg 7261 tcgagatcag cggcgttgag gacaggttca acgcttccct gggcacctac catgacctgc 7321 tcaagattat caaggacaag gacttcctcg acaacgagga gaatgaggac attcttgagg 7381 acatcgtgct gaccctgacc ctcttcgagg accgcgagat gatcgaggag aggctcaaga 7441 cctacgccca cctcttcgac gacaaggtga tgaagcaact gaagaggcgc aggtacactg 7501 gctggggcag gctttctagg aagctgatca acggcatcag ggacaagcag agcggcaaga 7561 cgatcctcga cttcctgaag tctgacggct tcgctaaccg caacttcatg cagctgatcc 7621 acgacgacag cctcaccttc aaggaggata tccagaaggc ccaggttagc ggccagggcg 7681 attctctgca tgagcatatc gctaacctcg ccggatcccc ggctatcaag aagggcatcc 7741 tgcagaccgt caagatcgtg gacgagctgg tgaaggtgat gggccacaag ccagagaaca 7801 tcgtgatcga gatggcgcgc gagaaccaga ccactcagaa gggccaaaag aacagccgcg 7861 agcgcatgaa gaggattgag gagggcatta aggagctggg cagccagatc ctcaaggagc 7921 acccagttga gaacacccag ctgcagaatg agaagctgta cctgtactac ctgcagaacg 7981 gcagggacat gtacgtggac caagagctgg acatcaacag gctgtccgac tacgacgttg 8041 accacatcgt gccgcagagc ttcatcaagg acgactccat cgacaacaag gtcctcaccc 8101 gcagcgacaa gaaccgcggc aagtctgaca acgtgccaag cgaggaggtc gtgaagaaga 8161 tgaagaacta ctggcggcag ctcctgaacg cgaagctcat cacccagcgc aagttcgaca 8221 acctgaccaa ggctgagaga ggcggcctga gcgagcttga taaggccggc ttcattaaga 8281 ggcagctggt cgagactagg cagatcacga agcacgtggc ccagatcctg gacagccgca 8341 tgaacaccaa gtacgacgag aacgacaagc tgatccgcga ggtgaaggtt atcaccctga 8401 agtccaagct ggtgagcgac ttccgcaagg atttccaatt ctacaaggtc cgcgagatca 8461 acaactacca ccacgcccac gacgcctacc tgaacgctgt tgttggcacc gccctgatca 8521 agaagtaccc aaagttggag agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc 8581 gcaagatgat cgccaagtcg gagcaagaga tcggcaaggc gaccgccaag tacttcttct 8641 acagcaacat catgaatttc ttcaagactg agatcaccct ggccaacggc gagatcagaa 8701 agaggccgct cattgagact aatggcgaga ctggcgagat cgtgtgggat aagggcaggg 8761 acttcgccac tgtgcgcaag gtgctgtcta tgccacaggt gaacatcgtt aagaagaccg 8821 aggtccagac cggcggcttc agcaaggagt ctatcctgcc gaagaggaac agcgataagc 8881 tgatcgcccg gaagaaggac tgggacccaa agaagtacgg cgggttcgat agcccaaccg 8941 tggcttactc tgtgctggtg gtggcgaagg tcgagaaggg caagtccaag aagctcaaga 9001 gcgtcaagga gcttctgggc atcaccatca tggagaggtc cagcttcgag aagaatccaa 9061 tcgatttcct tgaggccaag ggctacaagg aggtcaagaa ggacctcatc atcaagctgc 9121 caaagtactc cctgttcgag ttggagaacg gccgcaagag gatgctggct tctgctggcg 9181 agcttcaaaa gggcaacgag cttgccctgc cgtccaagta cgtcaacttc ctgtacctgg 9241 ccagccacta cgagaagctg aagggctctc cagaggacaa tgagcaaaag cagcttttcg 9301 tcgagcagca caagcactac ctggacgaga tcatcgagca gatcagcgag ttcagcaaga 9361 gggtgatcct ggccgacgcc aacctggata aggtgctcag cgcctacaac aagcaccgcg 9421 acaagccaat tcgcgagcag gccgagaaca tcatccacct gttcacgctg accaacctgg 9481 gtgccccagc agccttcaag tacttcgata ccaccatcga ccgcaagcgg tacacctcca 9541 ctaaggaggt tctggacgcc accctgatcc accagtctat cactggcctg tacgagacaa 9601 ggatcgacct ctcacagctg ggcggcgacc ctaagaagaa gaggaaggtt tgatgacccg 9661 ggtctccata ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt 9721 tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt 9781 ctatcaataa aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatcccccg 9841 aattaaggcc ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag 9901 aataacggat atttaaaact cgag //