LOCUS p02 8270 bp DNA circular 22-AUG-2017 DEFINITION . ACCESSION urn.local...2o-7x36jev KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers misc_feature 35..430 /label="U6gRNA" misc_feature 437..448 /label="Vector Identifier" misc_feature 457..1036 /label="CMV" misc_feature 1132..5253 /label="Cas9" misc_feature 5260..5328 /label="2A" misc_feature 5329..6051 /label="RFP" misc_feature 6076..6083 /Recognition_pattern="GTTT^AAAC" /annotation_group="GTTTAAAC" /label="PmeI, MssI" /note="Geneious type: restriction site" polyA_signal 6101..6325 /label="BGH pA" misc_feature 6498..7292 /label="KanR" rep_origin 7591..8264 /label="pUC ori" ORIGIN 1 gctgcttcgc gatgtacggg ccagatatac gcgtaaggtc gggcaggaag agggcctatt 61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat 121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt 181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt 241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgc 301 aggtggacac ctgcgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 361 caacttgaaa aagtggcacc gagtcggtgc ttttttctag gcccagcttt cttgtacaaa 421 gttggcatta gtcgacatcc aagcaacctt aattaatgac attgattatt gactagttat 481 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 541 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 601 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 661 gactatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 721 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 781 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg 841 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca 901 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt 961 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg 1021 gaggtctata taagcaacca ggttctggct aactagagaa cccactgctt actggcttat 1081 cgaaattaat acgactcact atagggagac ccaagctggc tagccgccac catggacaag 1141 aagtacagca tcggcctgga catcggcacc aactctgtgg gctgggccgt gatcaccgac 1201 gactacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc 1261 aagaagaacc tgatcggcgc cctgctgttc ggctctggcg aaacagccga ggccacccgg 1321 ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa 1381 gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag 1441 tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg 1501 gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa gaagctggcc 1561 gacagcaccg acaaggccga cctgagactg atctacctgg ccctggccca catgatcaag 1621 ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag 1681 ctgttcatcc agctggtgca gatctacaat cagctgttcg aggaaaaccc catcaacgcc 1741 agcagagtgg acgccaaggc catcctgagc gccagactga gcaagagcag acggctggaa 1801 aatctgatcg cccagctgcc cggcgagaag cggaatggcc tgttcggcaa cctgattgcc 1861 ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga ggatgccaaa 1921 ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc ccagatcggc 1981 gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc 2041 gacatcctga gagtgaacag cgagatcacc aaggcccccc tgtccgcctc tatgatcaag 2101 agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg 2161 cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatc 2221 gatggcggag ccagccagga agagttctac aagttcatca agcccatcct ggaaaagatg 2281 gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg gaagcagcgg 2341 accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca cgccattctg 2401 cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat cgagaagatc 2461 ctgaccttca gaatccccta ctacgtgggc cctctggcca ggggaaacag cagattcgcc 2521 tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga agtggtggac 2581 aagggcgcca gcgcccagag cttcatcgag cggatgacca acttcgataa gaacctgccc 2641 aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtacaacgag 2701 ctgaccaaag tgaaatacgt gaccgaggga atgcggaagc ccgcctttct gagcggcgag 2761 cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag 2821 ctgaaagagg actacttcaa gaaaatcgag tgcttcgaca gcgtggaaat cagcggcgtg 2881 gaagatcggt tcaacgcctc cctgggcgcc tatcacgatc tgctgaaaat tatcaaggac 2941 aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg 3001 acactgtttg aggaccgggg catgatcgag gaacggctga aaacctatgc ccacctgttc 3061 gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg caggctgagc 3121 cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg 3181 aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc 3241 tttaaagagg acatccagaa agcccaggtg tccggccagg gacactctct gcacgagcag 3301 atcgccaatc tggccggatc ccccgccatt aagaagggca tcctgcagac agtgaagatt 3361 gtggacgagc tcgtgaaagt gatgggccac aagcccgaga acatcgtgat cgaaatggcc 3421 agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc 3481 gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc 3541 cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg 3601 gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccacat tgtgccccag 3661 tccttcatca aggacgactc catcgataac aaagtgctga ctcggagcga caagaaccgg 3721 ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgc 3781 cagctgctga atgccaagct gattacccag aggaagttcg acaatctgac caaggccgag 3841 agaggcggcc tgagcgaact ggataaggcc ggcttcatta agcggcagct ggtggaaacc 3901 cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac 3961 gagaacgaca aactgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc 4021 gacttcagaa aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc 4081 cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg 4141 gaaagcgagt tcgtgtacgg cgattacaag gtgtacgacg tgcggaagat gatcgccaag 4201 agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac 4261 tttttcaaga ccgagatcac actggccaac ggcgagatca gaaagcggcc tctgatcgag 4321 acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc cacagtgcgg 4381 aaagtgctgt ccatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gaccggcggc 4441 ttcagcaaag agtctatcct gcccaagagg aactccgaca agctgatcgc cagaaagaag 4501 gattgggacc ctaagaagta cggcggcttt gacagcccca ccgtggccta ctctgtgctg 4561 gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg 4621 gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc 4681 aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc 4741 gagctggaaa acggccggaa gcggatgctg gcttctgccg gcgaactgca gaagggaaac 4801 gagctggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag 4861 ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac 4921 tacctggacg agatcatcga gcagattagc gagttctcca agcgcgtgat cctggccgat 4981 gccaacctgg acaaggtgct gagcgcctac aacaagcacc gggataagcc catcagagag 5041 caggccgaga atatcatcca cctgtttacc ctgaccaacc tgggagcccc tgccgccttc 5101 aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac 5161 gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag 5221 ctgggaggcg accccaagaa aaagcgcaaa gtggttaacg gcagtggaga gggcagagga 5281 agtctgctaa catgcggtga cgtcgaggag aatcctggcc cacgatcgat gcatgtgtct 5341 aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg 5401 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag 5461 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct 5521 accagcttca tgtacggcag cagaaccttc atcaaccaca cccagggcat ccccgacttc 5581 tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg 5641 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc 5701 aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc 5761 tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctggaagg cagaagcgac 5821 atggccctga agctcgtggg cgggggccac ctgatctgca acttcaagac cacatacaga 5881 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga ccacagactg 5941 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc 6001 agatactgcg acctccctag caaactgggg cacaaactta atgctgttaa ctgatgactc 6061 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag 6121 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact 6181 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt 6241 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat 6301 gctggggatg cggtgggctc tatggcttct actgggcggt tttatggaca gcaagcgaac 6361 cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga 6421 tggctttctt gccgccaagg atctgatggc gcaggggatc aagctctgat caagagacag 6481 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt 6541 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 6601 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 6661 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg 6721 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 6781 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 6841 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 6901 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 6961 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 7021 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga 7081 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 7141 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 7201 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 7261 ccttctatcg ccttcttgac gagttcttct gaattattaa cgcttacaat ttcctgatgc 7321 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catcaggtgg cacttttcgg 7381 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 7441 ctcatgagac aataaccctg ataaatgctt caataatagc acgtgctaaa acttcatttt 7501 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 7561 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 7621 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 7681 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 7741 agagcgcaga taccaaatac tgtctttcta gtgtagccgt agttaggcca ccacttcaag 7801 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 7861 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 7921 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 7981 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 8041 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 8101 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 8161 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 8221 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt //