LOCUS LV04 8671 bp DNA circular 19-MAR-1980 DEFINITION . ACCESSION urn.local...24-7x36jda SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT LSOWNER| COMMENT VNTNAME|LV04| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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12 0 0 400 0 0 0 0 7 48 2 18 COMMENT "Times New Roman") 0.8 0.7 0 COMMENT "HindIII (8181)" "@N (@S)" 65535 -6.39741 12 COMMENT 0 -10 2.63248 0.547009 #276#)) (CObjectList))) COMMENT (CObjectList)) COMMENT #278=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -694 100) COMMENT (CObjectList) (CObjectList)) COMMENT #279=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -2027 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 5 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 10 1917102304 (CObList))) FEATURES Location/Qualifiers misc_feature 468..503 /vntifkey="21" /label=Psi LTR complement(555..735) /vntifkey="19" /label=LTR_1 rep_origin 1513..1982 /vntifkey="33" /label=F1ori misc_feature 2099..2959 /vntifkey="21" /label=Amp rep_origin 3115..3777 /vntifkey="33" /label=pUC\ori polyA_signal 4500..4724 /vntifkey="25" /label=PolyA\signal LTR complement(4750..5005) /vntifkey="19" /label=LTR_2 CDS complement(5085..5786) /vntifkey="4" /label=tagBFP misc_feature 5787..5840 /vntifkey="21" /label=2A CDS complement(5859..6455) /vntifkey="4" /label=Puro misc_feature complement(6495..6994) /vntifkey="21" /label=hPGK /note="Geneious type: promoter uukaryotic" misc_feature 7027..7267 /vntifkey="21" /label=U6\Promoter /note="Geneious type: promoter uukaryotic" misc_feature complement(7266..7271) /Recognition_pattern="GAAGAC(2/6)" /vntifkey="21" /label=BbsI /note="Geneious type: restriction site" misc_feature 7280..7382 /vntifkey="21" /label=gRNA misc_feature 7296..7301 /Recognition_pattern="GAAGAC(2/6)" /vntifkey="21" /label=BbsI /note="Geneious type: restriction site" misc_feature 8487..8546 /vntifkey="21" /label=RRE misc_feature 4179..4213 /vntifkey="21" /label=PB-Left misc_feature 7553..7615 /vntifkey="21" /label=PB-Right misc_feature 7738..7797 /vntifkey="21" /label=cPPT BASE COUNT 2009 a 2235 c 2119 g 2308 t ORIGIN 1 tccctcatat ctcctcctcc aggtctgaag atcagcggcc ggccgcttgc tgtgcggtgg 61 tcttactttt gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct 121 ctatcctttg atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt 181 ctgatcctgt ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg 241 tttctaacag gccaggatta actgcgaatc gttctagctc cctgcttgcc catactatat 301 gttttaattt atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc 361 taattctccc ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc 421 tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt 481 cagcaagccg agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc 541 tgttcgggcg ccactgctag agattttcca cactgactaa aagggtctga gggatctcta 601 gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta 661 ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc 721 taaccagaga gacccagtac aggcaaaacg cgctgcttat atagacctcc caccgtacac 781 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt 841 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc 901 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa 961 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact 1021 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca 1081 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac 1141 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg 1201 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga 1261 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca 1321 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg 1381 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca 1441 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga 1501 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 1561 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 1621 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 1681 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 1741 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 1801 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 1861 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 1921 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt 1981 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 2041 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 2101 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 2161 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 2221 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 2281 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 2341 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 2401 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 2461 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 2521 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 2581 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 2641 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 2701 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 2761 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 2821 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 2881 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 2941 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 3001 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 3061 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 3121 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 3181 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 3241 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg 3301 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 3361 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 3421 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 3481 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 3541 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 3601 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 3661 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 3721 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 3781 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 3841 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 3901 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 3961 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 4021 cactcattag gcaccccagg ctttacactt tatgctcccg gctcgtatgt tgtgtggaat 4081 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc 4141 aattaaccct cactaaaggg aacaaaagct taatttaacc ctagaaagat agtctgcgta 4201 aaattgacgc atgcattctt gaaatattgc tctctctttc taaatagcgc gaatccgtcg 4261 ctgtgcattt aggacatctc agtcgccgct tggagctccc gtgaggcgtg cttgtcaatg 4321 cggtaagtgt cactgatttt gaactataac gaccgcgtga gtcaaaatga cgcatgatta 4381 tcttttacgt gacttttaag atttaactca tacgataatt atattgttat ttcatgttct 4441 acttacgtga taacttatta tatatatatt ttcttgttat agatatcatt aacggccgcc 4501 catagagccc accgcatccc cagcatgcct gctattgact tcccaatcct cccccttgct 4561 gtcctgcccc accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat 4621 ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac 4681 gggggagggg caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg 4741 ggtttaaact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 4801 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 4861 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 4921 agagagactc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgacttct 4981 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac 5041 agctgccttg taagtcattg gtcttaaagg tacctagcgg ccgctcaatt aagcttgtgc 5101 cccagtttgc tagggaggtc gcagtatctg gccactgcca cctcgtgctg ctcgacgtag 5161 gtctcgttgt tggcctcctt gattctttcc agtctgtagt ccacatagta gacgccaggc 5221 atcttgaggt tcttagcggg tttcttggat ctatatgtgg tcttgatgtt tgcgatcaga 5281 tggctcccgc ccacgagctt cagggccatg tcgtttctgc cttccaggcc gccgtcagcg 5341 gggtacagcg tctcggtgaa ggcctcccag ccgagtgttt tcttctgcat cacagggccg 5401 ttggatgtga agttcacccc tctgatcttg acgttgtaga tgaggcagcc gtcctggagg 5461 ctggtgtcct gggtagcggt cagcacgccc ccgtcctcgt atgtggtgac tctctcccat 5521 gtgaagccct cagggaagga ctgcttgaag aagtcgggga tgccctgggt gtggttgatg 5581 aaggtcttgc tgccgtagag gaagctagta gccaggatgt cgaaggcgaa ggggagaggg 5641 ccgccctcga ccaccttgat tctcatggtc tgggtgccct cgtagggctt gccttcgccc 5701 tcggatgtgc acttgaagtg atggttgtcc acggtgccct ccatgtacag cttcatgtgc 5761 atgttctcct taatcagctc gctcattggg ccaggattct cctccacgtc accgcatgtt 5821 aggagacttc ctctgccctc tcctccggac ccgccgccgg caccgggctt gcgggtcatg 5881 caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc 5941 tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac cccggcgcgc 6001 tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg 6061 ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg 6121 aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg 6181 ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc 6241 gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag 6301 agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg 6361 gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg 6421 gtggcgaggc gcaccgtggg cttgtactcg gtcatggtaa gcttcagctg ctcgagatct 6481 agatggatgc aggtcgaaag gcccggagat gaggaagagg agaacagcgc ggcagacgtg 6541 cgcttttgaa gcgtgcagaa tgccgggcct ccggaggacc ttcgggcgcc cgccccgccc 6601 ctgagcccgc ccctgagccc gcccccggac ccaccccttc ccagcctctg agcccagaaa 6661 gcgaaggagc aaagctgcta ttggccgctg ccccaaaggc ctacccgctt ccattgctca 6721 gcggtgctgt ccatctgcac gagactagtg agacgtgcta cttccatttg tcacgtcctg 6781 cacgacgcga gctgcggggc gggggggaac ttcctgacta ggggaggagt agaaggtggc 6841 gcgaaggggc caccaaagaa cggagccggt tggcgcctac cggtggatgt ggaatgtgtg 6901 cgaggccaga ggccacttgt gtagcgccaa gtgcccagcg gggctgctaa agcgcatgct 6961 ccagactgcc ttgggaaaag cgcctcccct acccggtaga attggatcgc caaggtcggg 7021 caggaagagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt 7081 agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga 7141 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac 7201 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg 7261 aaacagtctt catgcggatc cattagaatt catatgaaga ctttagagct agaaatagca 7321 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt 7381 ttgctttgtt actttataga agaaattttg agtttttgtt tttttttaat aaataaataa 7441 acataaataa attgtttgtt gaatttatta ttagtatgta agtgtaaata taataaaact 7501 taatatctat tcaaattaat aaataaacct cgatatacag accgataaaa cacatgcgtc 7561 aattttacgc atgattatct ttaacgtacg tcacaatatg attatctttc tagggttaag 7621 taacgcgtag ggcccggtac taaccaaact ggatctctgc tgtccctgta ataaacccga 7681 aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt 7741 atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaattg 7801 tggatgaata ctgccatttg tctgcagaat tggcgcacgc agtgccgatc cgttcactaa 7861 tcgaatggat ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc 7921 gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac 7981 tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat 8041 cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact 8101 tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc 8161 ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca 8221 ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac 8281 agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt 8341 taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag 8401 ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat 8461 accagacaat aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct 8521 tcctgctgct cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct 8581 ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac 8641 tttatattta tataattcac ttctccaatt g //