LOCUS LV03 9199 bp DNA circular 16-AUG-2017 DEFINITION . ACCESSION urn.local...1z-7x36jcx KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers misc_feature 1..6 /Recognition_pattern="C^CCGGG" /label="XmaI" /note="Geneious type: restriction site" misc_feature 1..6 /Recognition_pattern="CCC^GGG" /label="SmaI" /note="Geneious type: restriction site" misc_feature 7..1265 /label="EF1a Promoter" misc_feature 1266..1271 /Recognition_pattern="C^GTACG" /label="BsiWI" /note="Geneious type: restriction site" misc_feature 1278..1280 /label="Start codon" misc_feature 1284..1997 /label="GFP" misc_feature 2004..2009 /Recognition_pattern="A^CGCGT" /label="MluI" /note="Geneious type: restriction site" misc_feature 2732..2914 /label="LTR" misc_feature 4080..4940 /label="AmpR" misc_feature 6394..6576 /label="LTR" misc_feature 7878..8127 /label="U6 Promoter" misc_feature complement(8128..8134) /Recognition_pattern="CACCTGC(4/8)" /label="AarI" /note="Geneious type: restriction site" misc_feature 8135..8891 /label="LacZ" misc_feature 8892..8898 /Recognition_pattern="CACCTGC(4/8)" /label="AarI" /note="Geneious type: restriction site" misc_feature 8899..8974 /label="gRNA scaffold" ORIGIN 1 cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct 61 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat 121 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa 181 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg 241 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt 301 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg 361 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc 421 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt 481 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt 541 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat 601 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc 661 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca 721 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc 781 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg 841 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc 901 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac 961 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt 1021 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag 1081 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag 1141 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat 1201 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt 1261 cgtgacgtac ggccaccatg ggcagcgggg gcgaggagct gttcgccggc atcgtgcccg 1321 tgctgatcga gctggacggc gacgtgcacg gccacaagtt cagcgtgcgc ggcgagggcg 1381 agggcgacgc cgactacggc aagctggaga tcaagttcat ctgcaccacc ggcaagctgc 1441 ccgtgccctg gcccaccctg gtgaccaccc tctgctacgg catccagtgc ttcgcccgct 1501 accccgagca catgaagatg aacgacttct tcaagagcgc catgcccgag ggctacatcc 1561 aggagcgcac catccagttc caggacgacg gcaagtacaa gacccgcggc gaggtgaagt 1621 tcgagggcga caccctggtg aaccgcatcg agctgaaggg caaggacttc aaggaggacg 1681 gcaacatcct gggccacaag ctggagtaca gcttcaacag ccacaacgtg tacatccgcc 1741 ccgacaaggc caacaacggc ctggaggcta acttcaagac ccgccacaac atcgagggcg 1801 gcggcgtgca gctggccgac cactaccaga ccaacgtgcc cctgggcgac ggccccgtgc 1861 tgatccccat caaccactac ctgagcactc agaccaagat cagcaaggac cgcaacgagg 1921 cccgcgacca catggtgctc ctggagtcct tcagcgcctg ctgccacacc cacggcatgg 1981 acgagctgta cagggcttga tgaacgcgtt aagtcgacaa tcaacctctg gattacaaaa 2041 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 2101 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 2161 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 2221 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 2281 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 2341 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 2401 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc 2461 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 2521 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 2581 ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc 2641 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 2701 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 2761 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 2821 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 2881 acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat 2941 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 3001 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 3061 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 3121 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 3181 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 3241 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca 3301 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg 3361 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca 3421 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga 3481 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 3541 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 3601 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 3661 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 3721 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 3781 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 3841 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 3901 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt 3961 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 4021 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 4081 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 4141 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 4201 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 4261 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 4321 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 4381 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 4441 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 4501 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 4561 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 4621 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 4681 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 4741 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 4801 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 4861 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 4921 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 4981 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 5041 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 5101 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 5161 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 5221 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg 5281 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 5341 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 5401 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 5461 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 5521 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 5581 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 5641 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 5701 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 5761 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 5821 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 5881 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 5941 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 6001 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 6061 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc 6121 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca 6181 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag 6241 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga 6301 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat 6361 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga 6421 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 6481 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 6541 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 6601 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 6661 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 6721 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 6781 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 6841 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 6901 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 6961 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 7021 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 7081 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa 7141 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag 7201 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc 7261 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga 7321 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa 7381 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct 7441 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc 7501 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt 7561 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata 7621 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa 7681 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata 7741 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt 7801 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca 7861 accccgaggg gacccagaga gggcctattt cccatgattc cttcatattt gcatatacga 7921 tacaaggctg ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta 7981 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt 8041 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta 8101 tatatcttgt ggaaaggacg aaacaccgca ggtgaatacg caaaccgcct ctccccgcgc 8161 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg 8221 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta 8281 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca 8341 gctatgacca tgattacgga ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac 8401 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat 8461 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 8521 cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt 8581 cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgccc 8641 atctacacca acgtgaccta tcccattacg gtcaatccgc cgtttgttcc cacggagaat 8701 ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag 8761 acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg 8821 gtcggttacg gccaggacag tcgtttgccg tctgaatttg acctgagcgc atttttacgc 8881 gccggagaaa acacctgcgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg 8941 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt aagcttggcg taactagatc 9001 ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac 9061 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa 9121 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt 9181 tggcgccggc tcgaggggg //