LOCUS LV02 8321 bp DNA circular 16-AUG-2017 DEFINITION . ACCESSION urn.local...1u-7x36jcl KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers misc_feature 7..1264 /label="EF1a Promoter" misc_feature 1265..1270 /Recognition_pattern="C^GTACG" /label="BsiWI" /note="Geneious type: restriction site" misc_feature 1277..1876 /label="Puro" misc_feature 1333..1338 /Recognition_pattern="C^GTACG" /label="BsiWI" /note="Geneious type: restriction site" misc_feature 1877..1882 /Recognition_pattern="A^CGCGT" /label="MluI" /note="Geneious type: restriction site" misc_feature 2605..2787 /label="LTR" misc_feature 3953..4813 /label="AmpR" misc_feature 6267..6449 /label="LTR" misc_feature 7751..8000 /label="U6 Promoter" misc_feature 8021..8096 /label="gRNA scaffold" ORIGIN 1 cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct 61 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat 121 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa 181 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg 241 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt 301 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg 361 gttatggccc ttgcgtgcct tgaattactt ccactggctg cagtacgtga ttcttgatcc 421 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc 481 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg 541 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg 601 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca 661 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac 721 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca 781 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc 841 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc 901 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc 961 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta 1021 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg 1081 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt 1141 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc 1201 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc 1261 gtgacgtacg gccaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga 1321 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca 1381 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac 1441 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt 1501 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat 1561 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc 1621 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggagtctcgc ccgaccacca 1681 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg 1741 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg 1801 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg 1861 caagcccggt gcctgaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga 1921 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 1981 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 2041 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 2101 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 2161 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 2221 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 2281 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg 2341 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 2401 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc 2461 cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat 2521 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga 2581 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag 2641 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 2701 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 2761 tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag 2821 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct 2881 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 2941 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag 3001 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 3061 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 3121 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc 3181 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga 3241 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 3301 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 3361 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 3421 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 3481 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 3541 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 3601 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 3661 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 3721 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 3781 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga 3841 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 3901 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 3961 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 4021 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 4081 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 4141 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 4201 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 4261 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 4321 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 4381 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 4441 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 4501 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 4561 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 4621 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 4681 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 4741 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 4801 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 4861 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 4921 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 4981 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 5041 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 5101 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 5161 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 5221 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 5281 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 5341 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 5401 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 5461 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 5521 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 5581 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 5641 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 5701 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 5761 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 5821 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 5881 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 5941 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac 6001 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct 6061 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc 6121 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac 6181 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat 6241 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg 6301 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 6361 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 6421 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg 6481 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg 6541 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag 6601 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat 6661 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag 6721 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca 6781 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata 6841 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga 6901 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc 6961 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata 7021 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag 7081 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag 7141 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat 7201 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc 7261 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa 7321 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca 7381 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc 7441 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct 7501 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata 7561 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat 7621 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag 7681 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga 7741 ggggacccag agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg 7801 ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata 7861 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa 7921 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct 7981 tgtggaaagg acgaaacacc gnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc 8041 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt 8101 ttaagcttgg cgtaactaga tcttgagaca aatggcagta ttcatccaca attttaaaag 8161 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga 8221 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta 8281 cagggacagc agagatccac tttggcgccg gctcgagggg g //