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U6253 Sigma-Aldrich

Ubiquitin from bovine erythrocytes

BioUltra, ≥98% (SDS-PAGE), essentially salt-free, lyophilized powder

Synonym: ATP-dependent proteolytic factor, Ub

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Properties

Related Categories Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes, Cell Signaling and Neuroscience,
Quality Level   200
biological source   bovine erythrocytes
product line   BioUltra
assay   ≥98% (SDS-PAGE)
form   essentially salt-free, lyophilized powder
mol wt   8560 Da by calculation (monoisotopic mass)
  8565 Da by calculation (average mass)
extent of labeling   ≥5.4 mol glycine per mol protein
application(s)   western blot: suitable
storage temp.   2-8°C
Gene Information   bovine ... LOC(101902760)

Description

General description

A 76-amino acid protein (8.6 kDa) that is bound covalently by a conjugating enzyme to proteins that are targeted for degradation by 26S proteasome. Occurs in virtually all eukaryotes, including plants (thus, its name). The sequence is highly conserved; identical in animal sources from insects to humans.

Application

Ubiquitin from bovine erythrocytes can be used for in vitro ubiquitinylation assay. The product can also be used as a marker in western blotting.

Packaging

25 mg in poly bottle

5 mg in glass bottle

Biochem/physiol Actions

Ubiquitin is a small regulatory protein present in eukaryote tissues. Exogenous ubiquitin can stimulate apoptosis in numerous cell lines. E7 protein of human papilloma virus-16 stimulates Retinoblastoma Protein degradation via Ubiquitin-Proteasome Pathway.

Preparation Note

Ubiquitin from bovine erythrocytes can dissolved in water at 1 mg/ml to yield a clear, colorless solution.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Cellular Derived Blood Proteins and Enzymes

Cell damage is induced by reactive oxygen species (ROS). ROS are either free radicals, reactive anions containing oxygen atoms, or molecules containing oxygen atoms that can either produce free radic...
BioFiles 2006, 1.5, 4.
Keywords: Aerobic, Alzheimer Disease, Bacterial conjugations, Biofiles, Cancer, Metabolism, Oxidations, Parkinson Disease, Reductions, Respiration

Peer-Reviewed Papers
15

References

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