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RDD008 Sigma-Aldrich

Trizma® base

anhydrous, free-flowing, Redi-Dri, ≥99.9%

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

  • CAS Number 77-86-1

  • Linear Formula NH2C(CH2OH)3

  • Molecular Weight 121.14

  •  Beilstein/REAXYS Number 741883

  •  EC Number 201-064-4

  •  MDL number MFCD00004679

  •  PubChem Substance ID 329824219

  •  NACRES NA.25

Purchase

Properties

Quality Level   100
grade   anhydrous
description   aminopeptidase substrate
product line   Redi-Dri
assay   ≥99.9%
form   powder
quality   free-flowing
impurities   ≤0.2% Water (Karl Fischer)
color   white
pH   10.5-12
pH range   7 - 9
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg (lit.)
mp   167-172 °C (lit.)
solubility   water: soluble (Water < 0.2%)
absorption   ≤0.05 at 290 nm at 40%
λ   (A290 UV absorbance < 0.05)
  in 5 mM Tris; DNA (40% (w/w))
SMILES string   NC(CO)(CO)CO
InChI   1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key   LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Tris base has been used:
• To prepare Tris-HCl, which is used as a component of lysis buffer for protein characterization
• In the preparation of Tris-buffered saline Tween-20 (TBST) buffer for Western blotting
• In the preparation of TBS (Tris-buffered saline) that is used for dialysis to neutralize a protein
• As a component of nuclei extraction buffer

Packaging

1 kg in poly bottle

2.5 kg in poly drum

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Buffers - From Bnech to Bulk
Protocols & Articles

Articles

Enjoy the Free-flowing Experience - Redi-Dri™

What is the most frustrating moment you have experienced in the lab? Inadequate chemicals for the reaction? Or rock-solid material as you open your bottle when you only need a small amount? If you ha...
Keywords: Environmental

Protocols

Redi-Dri™ FAQs

A time study was performed to test the Red-Dri Packaging System against standard packaging. Equivalent units of both packaging systems containing potassium chloride (KCl) were introduced to a control...
Keywords: Absorption

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Redi-Dri™ – The world of free-flowing hygroscopic products

The Redi-Dri™ packaging system eliminates the common issue of clumping that is noted with many hygroscopic salts and buffers and provides free-flowing, ready-to- use material at all times. Don’t wast...
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Redi-Dri™ – 뭉치고 덩어리지지 않는 시약의 세계

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Peer-Reviewed Papers
15

References

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