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R2523 Sigma-Aldrich

REDTaq® ReadyMix PCR Reaction Mix

Complete PCR reagent with standard Taq DNA Polymerase and inert dye

  •  NACRES NA.55



Related Categories Core Bioreagents, Life Science Reagents for PCR, Molecular Biology, PCR Enzymes, PCR/Amplification,
Quality Level   200
form   liquid
feature   hotstart: no
color   red
Featured Industry   Agriculture
shipped in   wet ice
storage temp.   −20°C


General description

REDTaq® ReadyMix is a ready-to-use mixture of Taq DNA polymerase, 99% pure DNTPs, reaction buffer, and an inert red dye in a 2X concentrate. After the PCR reaction, the PCR product can be loaded directly onto an agarose gel. The red loading dye migrates at approximately the same rate as a 125 base pair fragment in a 1% agarose gel. The inert dye has no effect on the amplification process, and therefore, a sample can be easily re-amplified such as in “nested PCR”.


REDTaq® ReadyMix PCR Reaction Mix has been used:
• for routine polymerase chain reaction (PCR) amplification.
• for the PCR amplification of cDNA
• in semi-quantitative PCR

Features and Benefits

The benefits of REDTaq® with the convenience of ReadyMix
• Saves preparation time
• Reduces risk of contamination from multiple pipet steps and and increased reproducibility.
• Provides consistent reaction-to-reaction performance
• No loading buffer/tracking dye needs to be added. The PCR product is loaded directly into an agarose gel after amplification
• The red tracer migrates at approximately the same rate as a 125bp fragment (slightly faster than bromophenol blue)
• Amplifies targets up to 7 kb in length
• Because no loading buffer has been added, a sample can be easily re-amplifed such as in nested PCR.
• Stable at 4°C or −20°C.
• Ready-mix format reduces setup time.


Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL

Preparation Note

Add 25 μl of ReadyMix to template, primers and water to a final reaction volume of 50 μl. The PCR product is easily separated from the dye by standard purification methodologies. The inert red dye has no effect on automated sequencing and is suitable for restriction enzyme digestion, ligation and other downstream manipulations.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

REDTaq is a registered trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

NONH for all modes of transport
Protocols & Articles


History of PCR Discoveries - PCR Technologies Guide

Use the table of contents on the right to navigate to other sections of A Technical Guide to PCR Technologies.
A Technical Guide to PCR Technologies
Keywords: Amplification, Clinical, Degradations, Detection methods, Diagnostic, Diseases, Forensic, Gene expression, Genetic, Molecular biology, Nucleic acid denaturation, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Separation, Transcription

Improving Real-Time PCR Success

To meet increasing food supply demands of the global population, growers require seeds that can withstand the regional challenges found in their fields. In order to develop crop varieties that are mo...
Keywords: Agriculture, Amplification, Environmental, Polymerase chain reaction


Applications with REDTaq

Since its introduction, REDTaq has been well received by the scientific community. The original product idea was to add convenience to PCR by formulating a reaction that also contained loading buffer...
by Brian Ward and Keming Song
Sigma-Aldrich Corporation, St. Louis, MO, USA
Keywords: Amplification, Applications, Error, Melting, Methods, Nucleic acid annealing, Peptide synthesis, Polymerase chain reaction, Purification, Sequencing, transformation

PCR Protocol | Standard PCR Protocol

These steps are presented below in greater detail along with materials and reagent selection tips. This is a basic PCR protocol using Taq DNA polymerase.
Keywords: AGE, Amplification, Electrophoresis, Evaporation, Gel electrophoresis, PAGE, Polymerase chain reaction, Sequencing

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Agriculture | Genotyping

Sigma-Aldrich’s chemistry expertise can help customers develop and optimize their leaf and seed genotyping workflow to reduce cost per data point while increasing throughput rates. In addition, our g...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing, Whole genome amplification

PCR Master Mix

A PCR master mix, sometimes known as super mix or ready mix, is a batch mixture of PCR reagents at optimal concentrations that can be prepared and divided among many PCR tubes or 96-well PCR plates. ...
Keywords: Buffers, Gene expression, Nucleic acid hybridization, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Reductions, Transcription

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

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Sigma-Aldrich products are aligned to the discovery, development and production phases of the plant breeding workflow, allowing you to develop and produce new crop varieties faster. With materials ma...
Keywords: Building blocks, Mass spectrometry, Organic synthesis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Vitamins

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