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P3818 Sigma-Aldrich

Protein Disulfide Isomerase from bovine liver

>95% (SDS-PAGE), ≥100 units/mg protein, lyophilized powder

Synonym: PDI

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Properties

Related Categories 5.3.x.x Intramolecular oxidoreductases, 5.x.x.x Isomerases, Application Index, Biochemicals and Reagents, Cell Biology,
Quality Level   200
assay   >95% (SDS-PAGE)
form   lyophilized powder
specific activity   ≥100 units/mg protein
mol wt   107 kDa
composition   Protein, ~10% Lowry
storage temp.   −20°C

Description

General description

Protein disulfide isomerase (PDI) from bovine liver is a homodimer with a molecular weight of 107 kDa with the monomer corresponding to 57 kDa. The isoelectric point (pI) is approximately 4.2. The enzyme is a glycoprotein with 12% total carbohydrate content comprising of mannose, galactose, N-acetyl neuraminic acid (NANA) and 2-acetamido-2-deoxyglucose. PDI is an ubiquitous redox chaperone enzyme. It belongs to the, thioredoxin superfamily and is high conserved.

Application

Protein Disulfide Isomerase from bovine liver has been used:
• to study the functional role of PDI in parasite infection and the interaction between macrophage PDI and L. chagasi
• in the in vitro translation reaction for the generation of disulfide bonds
•  in insulin-disulfide reduction assay and peptide binding assay
• as a positive control in thiol-disulfide oxidoreductase activity assay

Packaging

0.1 mg in poly bottle

250 μg in glass bottle

Biochem/physiol Actions

Protein Disulfide Isomerase (PDI) is mainly located in the endoplasmic reticulum (ER), where it assists in protein-folding and thiol-disulfide exchanges. It aids protein refolding in vitro allowing recombinant proteins to achieve their native state.

Protein Disulfide Isomerase(PDI) has the C-terminal ER retention sequence Lys-Asp-Glu-Leu. It has active, intracellular traffic to different cell compartments. PDI supports internalization of Chlamydia, cholera and diphtheria toxins in some hosts. PDI is required for Sindbis virus infection and aids in reducing HIV gp120 protein thiols. PDI facilitates formation of the correct disulfide bonds by promoting rapid reshuffling of disulfide pairings.

Packaging

Package size based on protein content.

Physical properties

Protein Disulfide Isomerase (PDI) from bovine liver is a homodimer with a molecular weight of 107 kDa (gel filtration) and the molecular weight of the monomer has been reported at 57 kDA (SDS-PAGE). The enzyme is a glycoprotein with 12% total carbohydrate content, composed of 4.6% mannose, 2.5% galactose, 1.4% NANA, and 3.5% 2-acetamido-2-deoxyglucose.

Unit Definition

One unit cause a change in A650 of 0.01 per min of a 1.0 mg/mL solution of insulin in the presence of dithiothreitol at pH 7.5 at 25 °C.

Physical form

Lyophilized powder containing potassium phosphate buffer salts and stabilizer.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Mitochondrial Stress and ROS

Oxidative stress is implicated in the pathogenesis of lipotoxicity in both animal and human studies. Chronic oxidative stress is linked to insulin resistance in multiple tissues. Oxidative stress is ...
BioFiles 2011, 6.4, 22.
Keywords: Anti-inflammatory agents, Apoptosis, Cancer, Cell signaling, Cellular processes, Gene expression, Indicators, Metabolism, Oxidations, Respiratory

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Peer-Reviewed Papers
15

References

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