N3643 Sigma-Aldrich


Powder microbial growth medium

  •  NACRES NA.85



Related Categories All Liquid Media/Broths, Bacterial Culture Media, Broth / Media, Core Bioreagents, Escherichia coli & Coliforms,
Quality Level   200
grade   for molecular biology
sterility   non-sterile
form   powder
composition   Casamino Acids, 1 g/L
  MgSO4, 15 g/L
  NaCL, 5 g/L
  Tryptone, 10 g/L
  Yeast extract, 5 g/L
pH range   6.8 - 7.2 (2.2% solution)
Featured Industry   Food and Beverages
storage temp.   room temp
suitability   nonselective for Escherichia coli
  nonselective for coliforms


General description

NZCYM is a microbial growth medium used for the cultivation of E. coli and lambda bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.


Suitable for non-selective cultivation of lambda bacteriophage, and E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.


1 kg in poly bottle

250 g in poly bottle

Features and Benefits

NZCYM powder provides:
• Standard formulation
• Convenient package sizes
• A budget-friendly alternative to liquid


Stir to suspend 22 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Introduction to Microbial Media

Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins


Microbial Growth Protocols

From our library of Protocols, Sigma-Aldrich presents Microbial Growth Protocols
Keywords: Antibiotics

Peer-Reviewed Papers


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