N1142 Sigma-Aldrich

Neomycin solution

with 10 mg/mL neomycin in 0.9% NaCl, sterile-filtered, BioReagent, suitable for cell culture



Related Categories Aminoglycosides, Antibacterial, Antibiotics, Antibiotics A to Z, Antibiotics N-S,
Quality Level   200
biological source   Streptomyces fradiae
sterility   sterile-filtered
product line   BioReagent
form   liquid
potency   8-12 mg per mL
packaging   pkg of 20ML
concentration   10 mg/mL in 0.9% NaCl (neomycin)
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
color   clear
Mode of action   protein synthesis | interferes
UniProt accession no.   O14746
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
storage temp.   2-8°C
SMILES string   NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O)[C@H]2O[C@@H]3O[C@H](CO)[C@@H](O[C@H]4O[C@@H](CN)[C@@H](O)[C@H](O)[C@H]4N)[C@H]3O)[C@H](N)[C@@H](O)[C@@H]1O
Gene Information   human ... TERT(7015)


General description

Chemical structure: aminoglycoside


Neomycin solution has been used:
• as a supplement of autoclaved acidified water for bone marrow transplantation
• for the selection of stable clones
•  as a component of artificial sea water (ASWO) medium to remove bacteria from O. tauri cultures

Biochem/physiol Actions

Neomycin is an aminoglycoside antibiotic, produced by Streptomyces containing a minimum of 85% neomycin B. It acts as a selection agent for prokaryotic cells transformed using the neo selectable marker gene. Neomycin trisulfate is used to study the cytototoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts and extraction of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins. This product is recommended for use in cell culture applications at 5 mL/L.
Mode of action: This product acts by binding to the 30S and 50S subunits, causing miscoding and inhibiting initiation and elongation during protein synthesis. Neomycin also blocks voltage-sensitive Ca2+ channels without affecting the Na+/Ca2+ antiporter in neurons.
Antimicrobial spectrum: Neomycin acts against both gram-positive and gram-negative bacteria


This solution is formulated to contain 10 mg/mL neomycin in 0.9% sodium chloride. It is stable at 37 °C for 5 days and should be otherwise stored at 2-8°C.

Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic to determine the minimum concentration of an antibiotic that c...
Keywords: Antibiotics, Cell culture, Evaporation, Transfection

Related Content

Antibiotics Selection Guide

The Antibiotic Selection Guide enables researchers to spend more time on their research discoveries and less time seeking established antibiotic information. Link to hundreds of antibiotics for conta...
Keywords: Antibiotics, Cell biology, Cell culture, Genetic, High performance liquid chromatography, Reversed-phase chromatography

Peer-Reviewed Papers


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