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M8250 Sigma-Aldrich

MES hydrate

≥99.5% (titration)

Synonym: 2-(N-Morpholino)ethanesulfonic acid hydrate, 4-Morpholineethanesulfonic acid

  • CAS Number 1266615-59-1

  • Empirical Formula (Hill Notation) C6H13NO4S · xH2O

  • Molecular Weight 195.24 (anhydrous basis)

  •  MDL number MFCD00149409

  •  eCl@ss 32129211

  •  PubChem Substance ID 329818054

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffers A to Z, Good’s Buffers
Quality Level   300
  400
assay   ≥99.5% (titration)
form   crystalline powder
useful pH range   5.5 - 6.7
pKa    6.1
solubility   water: 0.25 g/mL, clear, colorless
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   O.OS(=O)(=O)CCN1CCOCC1
InChI   1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
InChI key   MIIIXQJBDGSIKL-UHFFFAOYSA-N

Description

General description

MES is one of the Good′s buffers, the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility, making it impermeable to membranes.

Application

MES hydrate has been used in the MS (Murashige and Skoog) media for growth of Arabidopsis seedlings. It has been added in the cytoskeleton buffer during immunofluorescence study.

Packaging

1 kg in poly bottle

25, 100, 250 g in poly bottle

25, 50 kg in poly drum

Preparation Note

A buffer using MES can be prepared by titrating with NaOH to the desired pH. Alternatively, stock solutions of MES and MES sodium salt can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare buffers of a given pH have been published. MES is not recommended for buffering at pH 7.4; other buffers should be considered.

Storage and Stability

Solutions are stable at 2-8°C for months. Sterilize by filtration through 0.2uM filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, and then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

Other Notes

Easily compare specifications for MES products with the MES specification table.

Solubility: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5 M or higher. This product is tested at 20g/80mL water, or 1.3 M. The pH of a solution should be between 2.5 and 5, depending on the concentration. A saturated solution at 0°C is approximately 0.65 M.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Buffers - From Bnech to Bulk

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Protocols & Articles

Protocols

Enzymatic Assay of Mutanolysin

This procedure may be used for Mutanolysin products. The Continuous Spectrophotometric Rate Determination (A600, Light path = 1 cm) is based on the following reaction:

Enzymatic assay of lipase type XIII from Pseudomonas sp. and Pseudomonas cepacia using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

To standardize a procedure for the enzymatic assay of Lipase Type XIII from Pseudomonas sp., Product No. L9518 , and Pseudomonas cepacia, using a coupled enzyme system of glycerol kinase and glycerop...
Keywords: Centrifugation, Extinction coefficient, High performance liquid chromatography, Size-exclusion chromatography

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Peer-Reviewed Papers
15

References

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