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LVCAS9NEO Sigma-Aldrich

Cas9 Neomycin Lenti Particles

  •  NACRES NA.51

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Properties

Related Categories CRISPR-Cas9, Cas9-only (DNA, mRNA, and virus), Functional Genomics and RNAi, Molecular Biology
Quality Level   200
packaging   vial of 200 μL
concentration   ≥1 x 10 6 VP/ml virus (via p24 assay)
application(s)   CRISPR: suitable
selection   neomycin / G418
shipped in   dry ice
storage temp.   −70°C

Description

General description

Ready-to-use Cas9 lentiviral particles are ideal for transducing a wide range of cell lines. The Cas9 Neomycin Lentiviral particles efficiently integrate Cas9 into the genomes of both dividing and non-dividing cell lines for use with CRISPR genome editing. Stably integrate Cas9 and Neo using the convenient, ready-to-use lentiviral particles while avoiding the tedious lentivirus production process.

Application

Functional Genomics/Screening /Target Validation/Genome Editing

Components

200 ul of purified lentiviral particles (minimum of 1 X 106 TU/ml via a p24 assay) expressing Cas9 only with blasticidin resistance.

Linkage

Click here to order gRNA plasmid and virus.

Safety & Documentation

Safety Information

RIDADR 
UN 3245 9
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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