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F4051 Sigma-Aldrich

Proteasome Fraction II from rabbit

5 mg/mL, Reticulocyte cell extract, solution

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Description

General description

Manufactured for Sigma by Boston Biochem., Inc.

Application

Proteasome Fraction II is ideal for demonstrating ubiquitin- and ATP-dependent degradation/conjugation of radiolabeled or immunodetectable substrates. Isopeptidase inhibitor and a proteasome inhibitor are highly recommended for the accumulation of ubiquitin protein conjugates. Typical assay concentration for FII is 0.5 - 4 mg/ml.

Biochem/physiol Actions

Essentially ubiquitin and ATP free. Contains E1, most E2s, UCHs, and the proteasome 20S and 26S isoforms.

Protein fraction of a cell extract that binds to anion-exchange resin.

Physical form

Solution in 25 mM HEPES, pH 7.6.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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