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E0526 Sigma-Aldrich

Endonuclease III from Escherichia coli

≥90% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, buffered aqueous glycerol solution, ≥10,000 units/mg protein

Synonym: Nth protein, Endo III



Related Categories Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes, Cell Signaling and Neuroscience,
Quality Level   200
biological source   Escherichia coli
recombinant   expressed in E. coli overproducing strain
assay   ≥90% (SDS-PAGE)
form   buffered aqueous glycerol solution
specific activity   ≥10,000 units/mg protein
mol wt   ≥23 kDa (211 amino acids)
application(s)   activity assay: suitable
shipped in   wet ice
storage temp.   −20°C
Gene Information   Escherichia coli K12 ... nth(947122)


Biochem/physiol Actions

Endo III, product of the nth gene, is an iron-sulfur containing DNA repair enzyme. It possesses DNA glycosylase activity with broad substrate specificity for mutated pyrimidine derivatives especially thymine glycol (Tg) but also for 5-hydroxycytosine and 5,6-dihydrothymine. In addition, it has an apurinic/apyrimidinic (AP) lyase activity which cleaves the DNA backbone by β elimination. Endo III protects E. coli cells from radiation damage caused by x-ray and UV irradiation and its activity overlaps the activity of endonuclease VIII. When overexpressed, it can also protect E.coli cells from alkylation defects caused by methylmethanesulphonate.

Unit Definition

One unit will cleave 50% of 0.5 pmol double strand oligonucleotide substrate containing 5,6-dihydrothymine in 15 min. at 30 °C.

Physical form

Solution in 20 mM Tris, pH 7.4, 100 mM NaCl, 1 mM DTT, 1 mM EDTA, and 50% glycerol.

Preparation Note

Purified in an iron bound form that confers the protein a light green color.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
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