Dulbecco’s Phosphate Buffered Saline Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

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Properties

Related Categories	Balanced Salt Mixtures and Solutions, Buffered Salt Solutions, Cell Culture, Cell Culture Media, Classical Media and Salts, Core Bioreagents, Dulbecco’s Phosphate Buffered Saline, Life Science Reagents for Cell Culture, Research Essentials More...
Quality Level	300
sterility	sterile-filtered
form	liquid
application(s)	cell culture \| mammalian: suitable
impurities	endotoxin, tested
components	glucose: no (Dextro) sodium pyruvate: no
shipped in	ambient

Description

Application

DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.

Dulbecco′s Phosphate Buffered Saline has been used for the dilution of fresh red blood cells to study the trapping of red blood cell aggregate. It has also been used to wash the wells of microtiter plate.

Safety & Documentation

Safety Information

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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SDS

Specification Sheet

D8537 - Formulation (74 KB)

Technical Article Jul01 (219 KB )

Technical Article Jul02 (305 KB )

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?

If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

How do I get lot-specific information or a Certificate of Analysis?

The lot specific COA document can be found by entering the lot number above under the "Documents" section.

What is the formulation for Product D8537, Dulbecco's Phosphate?

The formulation for 1 liter is 8 g Sodium Chloride, 0.2 g Potassium Phosphate, monobasic, 1.15 g Sodium Phosphate, dibasic, and 0.2 g Potassium Chloride.

IsProduct D8537, Dulbecco's Phosphate, a 1X or 10 X concentration?

This is a 1X concentration. The 10X concentration is listed under product D1408.

What is the pH ofa solution of Product D8537, Dulbecco's Phosphate?

The pH will be in the range of 7.1-7.5, according to the specification sheet.

How do I find price and availability?

There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.

What is the Department of Transportation shipping information for this product?

Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

What is a Salt Toxicity Test?

The Cell Culture assay is called a Salt Toxicity Test (previously called Cell Culture Salt Testing).A more complete description is below:Cells are harvested and counted. They are resuspended in DPBS and incubated at 37^°C, 5% CO2 for 1 hour. The cells are counted and %viability determined. The cells are then cultured for an additional 7 hours (total of 8 hours). The cells are counted again and the % viability determined. The viability at 1 hr has to be >95% of the original viability. The viability at 8 hrs must be >75% of the original viability.

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Protocols & Articles

Articles

3D Organoid Culture: New In Vitro Models of Development and Disease

Model systems drive biological research by recapitulating body processes and functions from the molecular to whole organism level. The human body is composed of both cellular and non-cellular materia...
Keywords: Adhesion, Cancer, Cell attachment, Cell culture, Cryopreservation, Diseases, Growth factors, Infectious Diseases, Processes and Functions, Vitamins

Bile Acids

The liver excretes excess cholesterol in the form of bile acids. Bile acids serve two purposes: to remove unwanted cholesterol from the body and to aid in lipid digestion in the intestine. Bile acids...
BioFiles 2007, 2.7, 17.
Keywords: Absorption, Digestions, Transcription

Protocols

Cell Culture Protocol 10: Testing Cells for Mycoplasma Contamination by Hoechst DNA Staining

DNA staining methods such as indirect Hoechst staining techniques are quick with results available within 72 hours which compares favorably with 4 weeks for mycoplasma detection by culture isolation....
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Culture media, Detection methods, Indicators, Polymerase chain reaction

Cell Culture Protocol 4: Subculture of Semi-Adherent Cell Lines

Some cell lines grow as a mixed population (e.g. B95-8 - marmoset) where a proportion of cells do not attach to the tissue culture flask and remain in suspension. Therefore, to maintain this heteroge...
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Culture media

Cell Culture Protocol 5: Subculture of Suspension Cell Lines

In general terms, cell lines derived from blood (e.g. lymphocytes) grow in suspension cultures. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastic cell lines). For these ...
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Centrifugation, Culture media, Growth factors

Counting cells using a hemocytometer - Cell Culture Protocol 6

For the majority of manipulations using cell lines, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to us...
ECACC Laboratory Handbook 4th Edition
Keywords: Carcinogens, Cell culture, Cryopreservation, Culture media, Flow cytometry

Cryopreservation of Cell Lines : Cell Culture Protocol 7

The protocol below describes the use of cell freezing methods involving an electric -80°C freezer for the cryopreservation of cell lines. ECACC routinely use a programmable rate-controlled freezer. T...
ECACC Laboratory Handbook 4th Edition
Keywords: Cryopreservation, Phase transitions

Human Skeletal Muscle Cells (HSkMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Induced Pluripotent Stem Cell Reprogramming Protocols

Introduction Methods Reprogramming of Human Fibroblasts using Non-Integrating Self-Replicating RNA Vectors Reprogramming of Peripheral Blood Mononuclear Cells (PBMCs) using STEMCCA Lentiviral...
Keywords: Apoptosis, Cell culture, Culture media, Gene expression, Growth factors, Immunocytochemistry, Polymerase chain reaction, Respiratory, Transduction, Transfection

Protocol: Human Follicle Dermal Papilla Cells Culture

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Rat Skeletal Muscle Cells (RSkMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Subculture of Adherent Cell Lines: Cell Culture Protocol 3

Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be subcultured or passaged in ord...
ECACC Laboratory Handbook 4th Edition
Keywords: Adhesion, Cell culture, Culture media

References

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