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D3687 Sigma-Aldrich

DirectLoad PCR 100 bp Low Ladder

ready-to-use marker for DNA electrophoresis



Related Categories Core Bioreagents, DNA Ladder Markers, Life Science Reagents for DNA/RNA Electrophoresis, Markers and Ladders, Molecular Biology,
Quality Level   200
grade   for molecular biology
form   liquid
usage   75 uses
suitability   suitable for electrophoresis (DNA)
storage temp.   −20°C


General description

Sigma’s DirectLoad PCR 100 bp Low Ladder contains 10 dsDNA fragments from 100 − 1,000 bases, evenly spaced in 100 bp repeats. Ladder should be loaded in a single lane on an agarose gel.


Suitable for size determination of PCR generated DNA fragments with either agarose or polyacrylamide gels.

Features and Benefits

• Ready-to-load
• Easy-to-use
• Popular band sizes


Sigma’s DirectLoad PCR 100 bp Low Ladder is provided in a solution of 5% glycerol, 4.2 mM EDTA, 0.09% orange G, and 0.0125% xylene cyanol.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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Markers and Ladders Selection Guide

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Antibody-Enzyme Mediated Hot Start PCR Protocol

During PCR assay preparation, nonspecific amplification can occur due to binding of PCR primers to nonspecific templates and from formation of primer dimers which result from using other primer molec...
Keywords: AGE, Amplification, Buffers, Electrophoresis, Enzyme activity, Gel electrophoresis, Nucleic acid denaturation, Polymerase chain reaction, Size-exclusion chromatography

Hot Start Taq Polymerase Protocol to Reduce Non-Specific Amplification

As PCR reactions sit at room temperature, during assay setup, nonspecific amplification can occur via:
Keywords: AGE, Amplification, Electrophoresis, Gel electrophoresis, Gene expression, Nucleic acid annealing, Polymerase chain reaction, Size-exclusion chromatography

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