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CRISPR16H Sigma-Aldrich

Lenti CRISPR HPRT1 gRNA Control Transduction Particles, High Titer (LV02 vector)

  •  NACRES NA.51



Related Categories CRISPR Controls (DNA and Virus), CRISPR-Cas9, Functional Genomics and RNAi, Molecular Biology
Quality Level   200
packaging   vial of 8x25 μL (aliquots)
concentration   (5x108 TU/ml (via p24 titering assay))
application(s)   CRISPR: suitable
shipped in   dry ice
storage temp.   −70°C


General description

The Lenti CRISPR HPRT1 gRNA Control Transduction Particles (LV02 vector) product is a critical positive control used to monitor transduction efficiency for lentiCRISPRs. The protein encoded by this gene is a transferase which plays a central role in the generation of purine nucleotides. Cells with intact HPRT1 will die upon exposure to 6-TG (Product No. A4882), an antimetabolite used in the treatment of leukemias. Conversely, cells with HPRT1 knocked out will increase over time with 6-TG selection. This targets a validated HPRT1 CRISPR site, which serves as an experimental control for lenti CRISPRs. The lenti CRISPR positive control uses a dual-component system consisting of a U6-driven guide RNA plasmid targeting the human HPRT1 gene and Ef1a-driven Puromycin resistance casette. This control requires Cas9 be delivered by a separate vector.

Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.


Functional Genomics/Screening/Target Validation

Other Notes

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.

Legal Information

CRISPR Use License Agreement

Lentiviral, WPRE, and Evrogen Licenses

Safety & Documentation

Safety Information

UN 3245 9
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles

Related Content


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