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CRISPR12 Sigma-Aldrich

CRISPR-Lenti Non-Targeting Control Plasmid

  •  NACRES NA.51

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Properties

Related Categories CRISPR Controls (DNA and Virus), CRISPR-Cas9, Functional Genomics and RNAi, Molecular Biology
Quality Level   200
packaging   vial of 50 μL
concentration   20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
application(s)   CRISPR: suitable
shipped in   dry ice
storage temp.   −20°C

Description

General description

The Sigma lenti CRISPR Non-Targeting Control is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. This vector is useful as a negative control in experiments using Sigma CRISPR lentiviral clones. The Sigma lenti CRISPR non-targeting control uses a one-plasmid system consisting of a EF1-alpha-driven Cas9, a U6-driven guide RNA (non-targeting), with both Puromycin and GFP co-expressed with Cas9.

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Also, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP001).

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas), originally found in bacteria and archaea, is employed by organisms to protect them from invading viruses and plasmids.

Application

Functional Genomics/Screening/Target Validation
• Non-targeting control, CRISPR lentivirus DNA format, for creating gene knockouts/genetic modifications in cell lines.

To see more application data, protocols, vector maps visit sigma.com/crisprs.

Features and Benefits

Serves as a non-targeting or negative experimental control (DNA format) for the CRISPR editing workflow using lenti CRISPRs. This allows you to examine the effect of transfection in your system with the Sigma lenti CRISPR/Cas9 plasmid.

Physical form

The Sigma CRISPR Lenti Non-Targeting Control Vector is provided as 1 μg of plasmid DNA in Tris-EDTA (TE).

Legal Information

CRISPR Use License Agreement

Lentiviral and WPRE License Agreements

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

CRISPR/Cas Nuclease RNA-guided Genome Editing

What is CRISPR/Cas9? How does CRISPR/Cas work? How does CRISPR Cas9 work? What is CRISPR/Cas9? Our role in developing CRISPR/Cas9
Keywords: Acetylations, Catalysis, Cell culture, Degradations, Gene expression, Genetic, PAGE, Polymorphisms, Transcription, Transduction, Transfection

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CRISPR

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Predictive Models for Neuroscience using CRISPR [VIDEO]

Caroline Beckett, the global CRISPR product manager, discusses reagent solutions for creating predictive models for neuroscience research. She notes that many neuroscientists want to be able to creat...
Keywords: Cell culture, Diseases, Neurodegenerative Diseases, Neuroscience

Peer-Reviewed Papers
15

References

Related Products

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