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CRISPR11 Sigma-Aldrich

CRISPR-Lenti Human Positive Control DNA

  •  NACRES NA.51

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Properties

Related Categories CRISPR Controls (DNA and Virus), CRISPR-Cas9, Functional Genomics and RNAi, Molecular Biology
Quality Level   200
packaging   vial of 50 μL
concentration   20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
application(s)   CRISPR: suitable
shipped in   dry ice
storage temp.   −20°C

Description

General description

This control is intended for use as a positive control for knockout of human gene targets when used with clones of the Sigma lenti CRISPRs. The DNA format of this control construct permits direct transfection in knockout experiments or use in the viral packaging process (for lentiviral-based knockdown experiments).

Validated CRISPR site, which serves as an experimental control for the Sigma lenti CRISPRs. A one-component positive control system consisting of a EF1-alpha-driven Cas9, a U6-driven guide RNA plasmid targeting the human EMX1 gene, with both Puromycin and GFP co-expressed with Cas9. The Sigma lenti CRISPR Human EMX1 Positive Control Vector is a lentiviral-based plasmid vector that contains a guide RNA sequence targeting the human EMX1 (empty spiracles homeobox 1) gene. EMX1 belongs to the EMX homeobox family. The vector backbone is the same as the Sigma lenti CRISPR products.

Application

Functional Genomics/Screening/Target Validation
• Positive control for creating gene knockouts/genetic modifications in cell lines.

To see more application data, protocols, vector maps visit sigma.com/crisprs.

Features and Benefits

Serves as an experimental control for the CRISPR editing workflow using lenti CRISPRs. Allows for validation of your system with the lenti CRISPR/Cas9 system. A positive result in a mis-match detection assay will indicate validation of your system.

Physical form

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Also, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP001). The human EMX1 positive Control Vector is provided as 1 ug of plasmid DNA in Tris-EDTA (TE).

Legal Information

CRISPR Use License Agreement

Lentiviral and WPRE License Agreements

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

CRISPR/Cas Nuclease RNA-guided Genome Editing

What is CRISPR/Cas9? How does CRISPR/Cas work? How does CRISPR Cas9 work? What is CRISPR/Cas9? Our role in developing CRISPR/Cas9
Keywords: Acetylations, Catalysis, Cell culture, Degradations, Gene expression, Genetic, PAGE, Polymorphisms, Transcription, Transduction, Transfection

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Predictive Models for Neuroscience using CRISPR [VIDEO]

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Peer-Reviewed Papers
15

References

Related Products

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