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CAS9NEO Sigma-Aldrich

Cas9 Neomycin Lenti Plasmid

  •  NACRES NA.51

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Properties

Related Categories CRISPR-Cas9, Cas9-only (DNA, mRNA, and virus), Functional Genomics and RNAi, Molecular Biology
Quality Level   200
recombinant   expressed in E. coli
packaging   vial of 50 μL
concentration   20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
application(s)   CRISPR: suitable
selection   blasticidin
shipped in   dry ice
storage temp.   −20°C

Description

General description

This product is a neomycin resistant lenti-Cas9 plasmid for generation of lentiviral particles. Use Sigma’s lentiviral Cas9 reagents to efficiently generate stable cell lines expressing Cas9 for CRISPR genome editing. Sigma’s lenti-Cas9 plasmid is one part of a two-vector CRISPR system with individual Cas9 and gRNA expression vectors. Please see the link above to order gRNA.

Application

Functional Genomics/Screening /Target Validation/Genome Editing

Features and Benefits

Use lentiviral particles to generate cells with stable expression of Cas9 nuclease.

Components

1ug of plasmid DNA, EF1a-Cas9-2A-Neomycin Lenti

Linkage

Click here to order gRNA plasmid and virus.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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