CAS9MRNA Sigma-Aldrich

Cas9 mRNA



Related Categories CRISPR-Cas9, Cas9-only (DNA, mRNA, and virus), Functional Genomics and RNAi, Molecular Biology
Quality Level   200
packaging   vial of 50 μL
concentration   500 ng/μL
shipped in   dry ice
storage temp.   −70°C


General description

Cas9 mRNA generated through in-vitro transcription which has been capped and polyA-tailed. This mRNA should be used in conjunction with purified guideRNA. The use of RNA vs. plasmid constructs avoids complications with promoter-embryo compatibility as well as the possibility of random integration of nuclease and gRNA-expressing plasmids into the host animal genome.


Functional Genomics/Transgenic Applications
• Creation of gene knockouts in transgenic applications
• Creation of knock-in animals with promoters, fusion tags or reporters integrated into endogenous genes

Features and Benefits

RNA ready to use in transgenic applications. Must be used in conjunction with a purified guideRNA sequence to mediate a site specific double strand break in the DNA.


1 vial containing 25ug of Cas9 mRNA.

Please note, product does not contain guideRNA sequence. This must be purchased separately through the Custom CRISPR product tab.


CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.

Physical form

Sigma Cas9 mRNA is supplied at a concentration of 500 ng/ul (50 ul Cas9 mRNA, capped and polyA-tailed)

Preparation Note

While Sigma implements the same high quality RNA synthesis procedures that have been successful in many mRNA-based ZFN transgenic projects, we highly recommend centrifuging your final, concentration-adjusted CRISPR RNA samples immediately prior to microinjection to ensure all particulate material has been removed which may result in clogging of microinjection needles.

Other Notes

Must be used in conjunction with the RNA format of a guideRNA sequence in order to mediate a double strand break in the DNA.

Typical microinjection concentrations used in the literature are in the ranges of 20-200 ng/ul for Cas9 mRNA and 10-50 ng/ul for gRNA.

While Sigma CRISPR RNA was not developed for robust application to cell culture, we have seen some success in detecting CEL-I activity using RNA-only formats for both CRISPR nucleases and paired nickases.  RNA-only delivery formats may be favorable for cell types which are sensitive to double stranded DNA such a dendritic cells or when promoter-cell incompatibilities exist.

Legal Information

CRISPR Use License Agreement

Safety & Documentation

Safety Information

NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


A CRISPR/Cas-GFP Vector for Rapid Expression Verification and Enrichment of Genome Edited Cells

In many genome editing experiments involving ZFNs and CRISPR/Cas nucleases, the first challenge is achieving successful delivery of plasmids and subsequent expression of the encoded nucleases. While ...
Keywords: Cloning, Gene expression, Sequencing, Transfection

CRISPR/Cas Nuclease RNA-guided Genome Editing

What is CRISPR/Cas9? How does CRISPR/Cas work? How does CRISPR Cas9 work? What is CRISPR/Cas9? Our role in developing CRISPR/Cas9
Keywords: Acetylations, Catalysis, Cell culture, Degradations, Gene expression, Genetic, PAGE, Polymorphisms, Transcription, Transduction, Transfection

Genome Editing in Plants with CRISPR/Cas9

Successful ZFN-induced gene targeting was published as early as 2003. Since that time targeted genome editing technology has rapidly advanced and been made commercially available. Most recently, the ...
Keywords: Cloning, Functional genomics, Gene expression, Genetic, Genomics, PAGE, Plant biotechnology, Recombination, Transcription, Transfection, transformation

Tips for Cell Engineering using Cas9-GFP CRISPR plasmids

CRISPR endonucleases have shown wide variation in their activity, even among multiple CRISPRs designed within close genomic proximity.1  For this reason, we highly recommend that you test 3 to 4 CRIS...
Keywords: Cell culture, Cloning, DNA purification, Gene expression, Microscopy, Purification, Reductions, Sequencing, Transcription, Transfection

Related Content


In recent years CRISPR has revolutionized gene editing capabilities, leading to sophisticated ways to create success with any experiment. As the first company to offer custom biomolecules globally fo...
Keywords: Genetic, Genomics, Transduction, Transfection

Predictive Models for Neuroscience using CRISPR [VIDEO]

Caroline Beckett, the global CRISPR product manager, discusses reagent solutions for creating predictive models for neuroscience research. She notes that many neuroscientists want to be able to creat...
Keywords: Cell culture, Diseases, Neurodegenerative Diseases, Neuroscience

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

CRISPR CRISPR/Cas9 Products and Services, Design and order CRISPR gRNA, Cas9, screening libraries, controls and companion products. Formats include plant, lentivirus, IVT-RNA, plasmid, synthetic, and protein.

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?