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C3805 Sigma-Aldrich

Cyclophilin A human

≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution

Synonym: CyP

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Properties

Related Categories 5.2.x.x Cis-Trans Isomerases, 5.x.x.x Isomerases, Analytical and Industrial Enzymes, Application Index, Biochemicals and Reagents,
Quality Level   200
biological source   human
recombinant   expressed in E. coli
assay   ≥95% (SDS-PAGE)
form   buffered aqueous solution
mol wt   20 kDa
concentration   ≥0.3 mg/mL
application(s)   cell culture | mammalian: suitable
storage temp.   −20°C
Gene Information   human ... PPIA(5478)

Description

General description

Cyclophilins are a family of extremely conserved proteins, known as immunophilins. Cyclophilin A (CyPA) is present in all tissues in prokaryotes and eukaryotes. It is present in all organs of human. Cyclophilin A (CyPA), a 20 kDa chaperone protein, that is liberated from vascular smooth muscle cells (VSMCs).

Application

Cyclophilin A human has been used in the study to interpret the mechanisms by which extracellular CyPA initiates endothelial activation.

Biochem/physiol Actions

Cyclophilin A (CyPA) participates in intracellular signalling and protein trafficking. It helps to control other proteins activity. CyPA plays a physiological and pathological role in cardiovascular diseases. Hence it acts as an important biomarker and mediator in several cardiovascular diseases, like vascular stenosis, atherosclerosis and abdominal aortic aneurysms. CyPA can induce the proliferation and inflammatory cell migration of vascular smooth muscle cells (VSMC) in vitro and in vivo.

Cyclophilins are peptidyl prolyl isomerases that catalyze the cis-trans isomerization of X-Pro peptide bonds. They are highly-conserved cytoplasmic enzymes that accelerate protein folding and facilitate HIV infectivity. Cyclosporin A binds to cyclophilin and inhibits its activity. The cyclosporin A-cyclophilin complex binds to calcineurin and inhibits T-cell activation. The structure of human, recombinant cyclophilin is given by Holzman, et al.

Physical form

Composed of amino acids 1-165 plus an N-terminal 20 amino acid His-tag

Solution in 20mM Tris, pH 8.0, containing 20 mM NaCl, 0.5 mM DTT and 10% glycerol

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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