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93350 Sigma-Aldrich

Trizma® base

puriss. p.a., ≥99.7% (T)

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

  • CAS Number 77-86-1

  • Linear Formula NH2C(CH2OH)3

  • Molecular Weight 121.14

  •  Beilstein/REAXYS Number 741883

  •  EC Number 201-064-4

  •  MDL number MFCD00004679

  •  PubChem Substance ID 57653537

  •  NACRES NA.25

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Properties

Related Categories Analytical Reagents, Analytical/Chromatography, Biochemicals and Reagents, Biological Buffers, Buffers A to Z,
Quality Level   200
description   aminopeptidase substrate
grade   puriss. p.a.
assay   ≥99.7% (T)
form   crystalline
ign. residue   ≤0.01% (as SO4)
pH   10.5-11.5 (25 °C, 10% in H2O)
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg (lit.)
mp   167-172 °C (lit.)
  168-172 °C
solubility   water: soluble (678 g/l at 20 °C)
anion traces   chloride (Cl-): ≤5 mg/kg
  sulfate (SO42-): ≤5 mg/kg
cation traces   Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   10 % in H2O
UV absorption   λ: 230 nm Amax: ≤0.6
  λ: 260 nm Amax: ≤0.05
  λ: 280 nm Amax: ≤0.04
  λ: 430 nm Amax: ≤0.01
SMILES string   NC(CO)(CO)CO
InChI   1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key   LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Tris base has been used:
• In the preparation of TAE buffer for TAE agarose gel used to run PCR products by gel electrophoresis
• As a component of sample buffer for SDS-PAGE
• In the preparation of IEC (ion-exchange chromatography) buffer

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
TY2900000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Buffers - From Bnech to Bulk

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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