Scepter 2.0 Handheld Automated Cell Counter

Cellular differentiation, the process by which progenitor cells differentiate into specialized cell types, is fundamental to developmental biology. In the immune system, immune progenitor cells must ...
Keywords: Diseases, Flow cytometry, Gene expression, Growth factors, Inflammation, Microscopy, Transcription

Assays to measure cellular proliferation, cell viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper ch...
Keywords: Cancer, Cell culture, Cell disruption, Cell proliferation, Click chemistry, Enzyme-linked immunosorbent assay, Flow cytometry, Glycolysis, Immunocytochemistry, Immunohistochemistry, Indicators, Microscopy, Nucleic acid denaturation, Reductions, Solvents, Western blot

During fermentation, carbohydrates present in the wort (a water-based solution of grain sugars) are converted by yeast into alcohol, carbon dioxide, and numerous byproducts. There are several stages ...
Keywords: Fermentation, Sample preparations, Size-exclusion chromatography

Peripheral blood mononuclear cells (PBMCs) are blood cells with round nuclei, such as monocytes and lymphocytes, with the lymphocyte population comprised of T cells, B cells, and natural killer (NK) ...
Keywords: Anticoagulants, Centrifugation, Clinical, Flow cytometry, Gene expression, Purification, Separation

By: Amedeo Cappione, Ph.D.1; Emily Crossley2; Nagaraja Thirumalapura, DVM, Ph.D.3; and Debra Hoover, Ph.D.1
Keywords: Antibiotics, Cell culture, Cell proliferation, Centrifugation, Clinical, Environmental, Flow cytometry, Fractionation, Gene expression, Immobilization, Immunology, Indicators, Inflammation, Infrared spectroscopy, Microbiology, Sample preparations, Separation

Much research has recently focused on elucidating the mechanisms by which eukaryotic cells regulate their size with respect to growth rate and cell cycle stage. These mechanisms are known to differ b...
Keywords: Apoptosis, Cell culture, Cell proliferation, Flow cytometry, High performance liquid chromatography, Sample preparations

Micron-sized beads are used in a variety of biological applications, ranging from daily validation of flow cytometer performance, to purification of fusion protein constructs from cell lysates. Depen...
Keywords: Immunoprecipitation, Purification, Sample preparations

Apoptosis, the process of programmed cell death, is an essential component of many biological processes. Initial studies revealed that reduction in cell volume was an early morphological change durin...
Keywords: Apoptosis, Biological processes, Degradations, Flow cytometry, Microscopy, Reductions

Obesity is a prevalent health risk in many industrialized nations, and its occurrence is closely associated with a number of pathological disorders, such as non-insulin dependent diabetes, hypertensi...
Keywords: Cancer, Cell culture, Diabetes, Microscopy, Obesity

Mastitis is an inflammatory change in the mammary gland most frequently characterized by pathological changes in mammary tissue. This potentially fatal infection is the most common and costly disease...
Amedeo Cappione, Ph.D. and Janet Smith
MilliporeSigma
Keywords: Centrifugation, Clinical, Culture media, Detection methods, Flow cytometry, Microscopy, Sample preparations, Separation

Cultured cells have proved to be convenient, powerful model systems for elucidating biological processes and for testing the safety, effcacy and mechanism of therapeutic candidates. The main objectiv...
Keywords: Apoptosis, Biological processes, Cell culture, Centrifugation, Microscopy, Transcription

Solid tumors grow in a three-dimensional (3D) spatial conformation, resulting in a heterogeneous exposure to oxygen and nutrients as well as to other physical and chemical stresses. To mimic the 3D s...
Keywords: Cancer, Cell culture

How do you assess the state of your cell culture? We’ve got some best practices to share.  
Keywords: Cell culture