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PHCC20060 Millipore

Scepter 2.0 Handheld Automated Cell Counter

Rapid cell counts in a handheld easy to use format, includes pkg of 60 μm Scepter Cell Counter Sensors

  •  eCl@ss 32011202

  •  NACRES NB.22



mfr. no.   Scepter
application(s)   cell analysis: suitable (cell counting)
particle size   6-36 μm
compatibility   for use with cells sized 4.0–25.0 μm
shipped in   ambient


General description

The Scepter 2.0 handheld automated cell counter provides a fast and convenient method for counting cells and particles and is compatible with both the 40 and 60uM sensor. The system employs the Coulter principle in a miniaturized, handheld, format enabling rapid cell counting; what used to take 10 minutes now takes less than 30 seconds. The user prepares a dilution of the cell culture of interest and uses the Scepter cell counter to aspirate a sample of this dilution into the Scepter sensor.

The Scepter cell counter’s screen displays:

Cell concentration
Average cell size
Average cell volume
Histogram of size or volume distribution
This system is intended for research use only and has been tested with cell types representative of those in use today.

The Scepter Cell Counter delivers rapid cell counts in a handheld easy to use format. Its on screen instructions guide you through the sampling process and in less than 40 seconds reveil data on cell concentration, average diameter, average volume and a histogram displaying population information on your culture. The device stores up to 72 counts which can be downloaded to your computer and uploaded to Excel for further manipulation.
FREE PACK OF 60μM Sensors included.


Rapid cell counts in a handheld easy to use format, includes pkg of 60 μm Scepter Cell Counter Sensors

Research Category
Cell Culture


1 Scepter Cell Counter

1 Test Bead Vial

1 USB Cable

1 Downloadable Software

2 O-rings

50 Sensors (60µM)

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Assessing Enriched Murine CD4+ T Cells Differentiated Towards Effector T Helper Cell Lineages

Cellular differentiation, the process by which progenitor cells differentiate into specialized cell types, is fundamental to developmental biology. In the immune system, immune progenitor cells must ...
Keywords: Diseases, Flow cytometry, Gene expression, Growth factors, Inflammation, Microscopy, Transcription

Cell Viability and Proliferation Assays

Assays to measure cellular proliferation, cell viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper ch...
Keywords: Cancer, Cell culture, Cell disruption, Cell proliferation, Click chemistry, Enzyme-linked immunosorbent assay, Flow cytometry, Glycolysis, Immunocytochemistry, Immunohistochemistry, Indicators, Microscopy, Nucleic acid denaturation, Reductions, Solvents, Western blot

Counting Yeast Cells for Brewing and Wine Industries

During fermentation, carbohydrates present in the wort (a water-based solution of grain sugars) are converted by yeast into alcohol, carbon dioxide, and numerous byproducts. There are several stages ...
Keywords: Fermentation, Sample preparations, Size-exclusion chromatography

Human PBMC Isolation and Counting Using Scepter™ 2.0 Handheld Automated Cell Counter

Peripheral blood mononuclear cells (PBMCs) are blood cells with round nuclei, such as monocytes and lymphocytes, with the lymphocyte population comprised of T cells, B cells, and natural killer (NK) ...
Keywords: Anticoagulants, Centrifugation, Clinical, Flow cytometry, Gene expression, Purification, Separation

Immunomonitoring using the Scepter™ 2.0 Cell Counter and Scepter™ Software Pro

By: Amedeo Cappione, Ph.D.1; Emily Crossley2; Nagaraja Thirumalapura, DVM, Ph.D.3; and Debra Hoover, Ph.D.1
Keywords: Antibiotics, Cell culture, Cell proliferation, Centrifugation, Clinical, Environmental, Flow cytometry, Fractionation, Gene expression, Immobilization, Immunology, Indicators, Inflammation, Infrared spectroscopy, Microbiology, Sample preparations, Separation

Novel and Rapid Method to Assess Proliferative Properties of Mammalian Cells by Monitoring Cell Size Distributions

Much research has recently focused on elucidating the mechanisms by which eukaryotic cells regulate their size with respect to growth rate and cell cycle stage. These mechanisms are known to differ b...
Keywords: Apoptosis, Cell culture, Cell proliferation, Flow cytometry, High performance liquid chromatography, Sample preparations

Precise and Accurate Bead Counting Using the Scepter™ 2.0 Handheld Automated Cell Counter

Micron-sized beads are used in a variety of biological applications, ranging from daily validation of flow cytometer performance, to purification of fusion protein constructs from cell lysates. Depen...
Keywords: Immunoprecipitation, Purification, Sample preparations

Predicting Cell Death by Rapidly Assessing Size Changes with the Scepter™ Cell Counter

Apoptosis, the process of programmed cell death, is an essential component of many biological processes. Initial studies revealed that reduction in cell volume was an early morphological change durin...
Keywords: Apoptosis, Biological processes, Degradations, Flow cytometry, Microscopy, Reductions

Rapid Analysis of Human Adipose-Derived Stem Cells and 3T3-L1 Differentiation Towards Adipocytes

Obesity is a prevalent health risk in many industrialized nations, and its occurrence is closely associated with a number of pathological disorders, such as non-insulin dependent diabetes, hypertensi...
Keywords: Cancer, Cell culture, Diabetes, Microscopy, Obesity

Rapid Counting of Somatic Cells in Dairy Milk Using the Scepter™ 2.0 Cell Counter

Mastitis is an inflammatory change in the mammary gland most frequently characterized by pathological changes in mammary tissue. This potentially fatal infection is the most common and costly disease...
Amedeo Cappione, Ph.D. and Janet Smith
Keywords: Centrifugation, Clinical, Culture media, Detection methods, Flow cytometry, Microscopy, Sample preparations, Separation

Three-dimensional culture and assessment of drug-induced cell death using the CellASIC® ONIX Microfluidic Platform

Cultured cells have proved to be convenient, powerful model systems for elucidating biological processes and for testing the safety, effcacy and mechanism of therapeutic candidates. The main objectiv...
Keywords: Apoptosis, Biological processes, Cell culture, Centrifugation, Microscopy, Transcription


Cancer Stem Cell Tumorsphere Formation Protocol

Solid tumors grow in a three-dimensional (3D) spatial conformation, resulting in a heterogeneous exposure to oxygen and nutrients as well as to other physical and chemical stresses. To mimic the 3D s...
Keywords: Cancer, Cell culture

Related Content

Cell Analysis Best Practices [VIDEO]

How do you assess the state of your cell culture? We’ve got some best practices to share.  
Keywords: Cell culture

Peer-Reviewed Papers


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