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  • 568323-U - Discovery® BIO Wide Pore C8 HPLC Column

568323-U Supelco

Discovery® BIO Wide Pore C8 HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

  •  eCl@ss 32110501

  •  NACRES SB.52



Related Categories 5 micron Discovery BIO Wide Pore (300) C8 Columns, Analytical/Chromatography, C8 HPLC Columns, Discovery BIO, Discovery BIO Wide Pore (300 Å) C8,
Quality Level   100
feature   endcapped
mfr. no.   Discovery
extent of labeling   5% Carbon loading
parameter   0-70 °C temperature
  400 bar pressure (5801 psi)
application(s)   HPLC: suitable
L × I.D.   25 cm × 4.6 mm
surface coverage   4 μmol/m2
matrix   silica particle platform
matrix active group   C8 (octyl) phase
particle size   5 μm
pore size   300 Å
surface area   100 m2/g
suitability   suitable for L7 per USP
separation technique   reversed phase


General description

Discovery BIO Wide Pore C8 exhibits hydrophobicity intermediate between the Discovery BIO Wide Pore C5 and the Discovery BIO Wide Pore C18. The difference in hydrophobicity gives it unique selectivity relative to these other phases. It is ideal for peptide mapping because it provides complementary information compared to a C18 separation. Because of its intermediate hydrophobicity, we also recommend it for method development or scouting work. As with all Discovery BIO Wide Pore phases, the C8 phase gives efficient, symmetrical peaks, exceptional stability, long column lifetime, and LC-MS compatibility.

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Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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