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L3141 Sigma-Aldrich

Lectin from Cicer arietinum (chick pea)

lyophilized powder

Synonym: Cicer arietinum agglutinin

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Properties

form   lyophilized powder
potency   <20 μg per mL agglutination activity
composition   Protein, ~80% biuret
storage temp.   −20°C

Description

Biochem/physiol Actions

Cicer arietinum agglutinin is not blood group specific but agglutinates human erythrocytes that have undergone treatment with papain. In addition, the lectin has an affinity for glycoproteins like fetuin and IgM.

Quality

Purified.

Physical form

Lyophilized powder containing sodium acetate and sodium chloride

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution using phosphate buffered saline, pH 6.8. This activity is the lowest concentration to agglutinate a 2% suspension of papain-treated human erythrocytes after 1 hr incubation at 25 °C.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Bacterial Transformation

Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. It was first reported in Streptococcus pneu...
Keywords: Antibiotics, Cloning, Environmental, Gene expression, Genetic, Molecular biology, Peptide synthesis, Polymerase chain reaction, Transfection, transformation

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