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CMC0012 Sigma-Aldrich

XLDNA SIG10 Electrocompetent cells

for BAC & cosmid cloning

  •  NACRES NA.85

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Properties

Related Categories Cloning and Expression, Molecular Biology, Transformation of Bacteria More...
grade   for molecular biology
cell transformation   competent cell type: electrocompetent
transformation efficiency: ≥1 x 1010 cfu/μg
shipped in   dry ice
storage temp.   −70°C

Description

General description

The XLDNA SIG10 Electrocompetent cells are designed for transformation of large constructs and increase in DNA yields.
These cells are manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts and are an excellent choice for large construct transformation

Genotype

F - pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 F80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupG λ- tonA (StrR)

Features and Benefits

• Cells that enable the most challenging library construction and screening.
• Highest available transformation efficiencies for library construction and screening.
• Optimized for high yields of DNA

Components

• XLDNA SIG10 electrocompetent cells
• pUC 19 transformation control DNA
• recovery medium for cloning


Safety & Documentation

Safety Information

RIDADR 
UN 3245 9

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Competent Cell Selection & E. coli Markers Guide

We offer a range of Escherichia coli bacterial cells made competent with the highest efficiencies by optimized procedure specific to each strain. Choose from 24 new competent cells for a wide variety...
Keywords: Bacterial conjugations, Cloning, Gene expression, Methylations, Recombination, Transcription, transformation

Molecular Biology Handbook

Molecular Biology is the science that aims to understand biological activity at the molecular level. These biological activities usually involve the plant or animal cell, and the nucleic acids and pr...
Keywords: Cell culture, Cell signaling, Cloning, Detection methods, Electrophoresis, Gel electrophoresis, Gene expression, Genetics, Molecular biology, Molecular biology techniques, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction - quantitative, Protein electrophoresis, Purification, Transfection, Western blot, transformation

Protocols

Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

XLDNA V2 Electrocompetent Cells

Large-insert DNA cloning and BAC library construction demand the highest transformation efficiency and recovery of the largest clones possible. Sigma’s XLDNA V2 Electrocompetent cells were developed ...
Keywords: Antibiotics, Cloning, Peptide synthesis, transformation

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L3022 LB Broth (Lennox), Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.
L2897 LB Broth with agar (Lennox), Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.
L5542 LB Agar Plates, pre-poured LB agar plates for E. coli
CMR0002 Recovery Medium for Cloning, for use with competent cells

Technical Service:

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