• USA Home
  • C3175 - Chloramphenicol-Water Soluble

C3175 Sigma-Aldrich

Chloramphenicol-Water Soluble

powder, BioReagent, suitable for cell culture

  •  NACRES NA.76



Related Categories Antibiotics, Antibiotics A to Z, Antibiotics  A-F, Biochemicals and Reagents, Cell Culture,
product line   BioReagent
form   powder
application(s)   cell culture | mammalian: suitable
solubility   H2O: 50-500 mg/mL (stock solution)
  PBS: 50-500 mg/mL (stock solution)
  other salt solutions: not recommended
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
storage temp.   room temp



100 mg in glass bottle


Recommended for use in cell culture applications at approximately 5 μg/ml of active component (chloramphenicol).

Biochem/physiol Actions

Mode of Action: Inhibits elongation of peptidyl transferase.


Package size based on chloramphenicol

Other Notes

Formulated to contain approx. 100 mg chloramphenicol per gram of powder; balance 2-hydroxypropyl-β-cyclodextrin.

Keep container tightly closed in a dry and well-ventilated place. Light sensitive. Keep in a dry place.

Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How do I calculate the molarity ofproduct C3175, Chloramphenicol-Water Soluble?
Product C3175 (Chloramphenicol-Water Soluble)is a complex of chloramphenicol and cyclodextrin. Cyclodextrin is used to aid in the solubility of chloramphenicol into water. In order to prepare a molar solution of chloramphenicol you will have to base it on the chloramphenicol content in the solution and not the entire complex. Product C3175 is formulated to contain approximately 100mg chloramphenicol per gram of powder. Based onthis average,if you are preparing a 500 mg/ml stock solution, there will be approximately 50 mg of chloramphenicolin 1 ml. Below is the calculated molarity ofchloramphenicol when the complex is dissolved at 500 mg/ml in water.molecular weightX unknown molarity X volume = complex weight X complex ratio323.13 g/mol X Molarity X 1 ml = 500 mg X 0.1 Molarity= 0.155 Mchloramphenicol molecular weight = 323.13 g/molmolarity (unknown) = mol/Lvolume = 1mlcomplexweight = 500 mgcomplex ratio = 100 mg chloramphenicol/1 gm complex
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions
Protocols & Articles


Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic to determine the minimum concentration of an antibiotic that c...
Keywords: Antibiotics, Cell culture, Evaporation, Transfection

Related Content

Antibiotics Selection Guide

The Antibiotic Selection Guide enables researchers to spend more time on their research discoveries and less time seeking established antibiotic information. Link to hundreds of antibiotics for conta...
Keywords: Antibiotics, Cell biology, Cell culture, Genetic, High performance liquid chromatography, Reversed-phase chromatography

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?