• USA Home
  • A9528 - Amphotericin B solubilized

A9528 Sigma-Aldrich

Amphotericin B solubilized

powder, γ-irradiated, BioXtra, suitable for cell culture

Synonym: Amphotericin B from Streptomyces sp., Fungizone

  • CAS Number 1397-89-3

  • Empirical Formula (Hill Notation) C47H73NO17

  • Molecular Weight 924.08

  •  Beilstein/REAXYS Number 78342

  •  MDL number MFCD00877763

  •  eCl@ss 34010400

  •  PubChem Substance ID 24891468



Related Categories Antibiotics, Antibiotics A to Z, Antibiotics by Mechanism of Action, Antibiotics  A-F, Antifungal,
Quality Level   300
sterility   γ-irradiated
product line   BioXtra
form   powder
application(s)   cell culture | mammalian: suitable
Mode of action   cell membrane | interferes
antibiotic activity spectrum   fungi
storage temp.   2-8°C
SMILES string   C[C@H]1O[C@@H](O[C@@H]2C[C@@H]3O[C@](O)(C[C@@H](O)C[C@@H](O)[C@H](O)CC[C@@H](O)C[C@@H](O)CC(=O)O[C@@H](C)[C@H](C)[C@H](O)[C@@H](C)\C=C\C=C\C=C\C=C\C=C\C=C\C=C\2)C[C@H](O)[C@H]3C(O)=O)[C@@H](O)[C@@H](N)[C@@H]1O
InChI   1S/C47H73NO17/c1-27-17-15-13-11-9-7-5-6-8-10-12-14-16-18-34(64-46-44(58)41(48)43(57)30(4)63-46)24-38-40(45(59)60)37(54)26-47(61,65-38)25-33(51)22-36(53)35(52)20-19-31(49)21-32(50)23-39(55)62-29(3)28(2)42(27)56/h5-18,27-38,40-44,46,49-54,56-58,61H,19-26,48H2,1-4H3,(H,59,60)/b6-5+,9-7+,10-8+,13-11+,14-12+,17-15+,18-16+/t27-,28-,29-,30+,31+,32+,33-,34-,35+,36+,37-,38-,40+,41-,42+,43+,44-,46-,47+/m0/s1


General description

Chemical structure: polyene


1, 5 g in glass bottle

50, 100, 500 mg in glass bottle


Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.

Biochem/physiol Actions

Amphotericin B binds to sterols, forming pores in the membrane, and causing small molecules to leak out. The product is most effective against fungi and yeast.


Solubilized Amphotericin B is packaged in amber glass bottle, with the contents inside an inserted fused cone.


This product is a colloidal suspension of Amphotericin B, using deoxycholate as the solubilizing agent. The formulation is ~45% Amphotericin B and 35% sodium deoxycholate in sodium phosphate and sodium chloride.


Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years.

Preparation Note

Amphotericin B is soluble at 19.6-20.4 mg/ml in water, at natural pH without heating, giving a yellowish clear to slightly hazy solution.
Aqueous solutions cannot be sterile filtered due to poor solubility. If reconstituted in sterile water, there is no need to filter sterilize.

It is also soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL).

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Product is sensitive to light and moisture. Keep in a dry place.

The actual solid weight of the packaged product is calculated according to the UV purity.

Kit component only


Product #

Add to Cart

Amphotericin B ~ 45 %    
Sodium deoxycholate ~ 35 %    
Sodium phosphate balance    
Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Target organs 
Respiratory system
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the shelf life of Product A9528, Amphotericin B solubilized?
Product No. A9528 has a recommended retest date of 5 years from the initial QC release date.This date can be found on the lot-specific Certificate of Analysis.
What is the amount of Amphotericin B in Product No. A9528?
The package size is the amount of Amphotericin B present in the vial.If weighing the product, utilize the potency value on the lot specific Certificate of Analysis as a correction factor.
What concentration should Product A9528, Amphotericin B solubilized, be used at?
We recommend to use Product No. A9528 at 5.6 mg solid per Liter.Cell culture tested amphotericin B solubilized preparation is approximately 45% amphotericin B, 35% sodium deoxycholate, with the balance being sodium phosphate. The amphotericin B is at least 80% amphotericin B and 3-5% amphotericin A by HPLC.The exact amount of amphotericin B in the product can be found on the lot specific Certificate of Analysis under Potency.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions
Protocols & Articles


Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic to determine the minimum concentration of an antibiotic that c...
Keywords: Antibiotics, Cell culture, Evaporation, Transfection

Human Microbiome Research Workflow: An Overview of the Role in Human Health and Disease

Previously, the study of human microbiology was based on identification, isolation and culture of single microbes, such as bacteria, fungi and viruses. Scientists collected these organisms from patie...
Keywords: Amplification, Antibiotics, Antifungals, Cell culture, Cell disruption, Clinical, Diabetes, Digestions, Diseases, Environmental, Gas chromatography, Gas chromatography mass spectrometry, Gastrointestinal, Genetics, Genomics, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Metabolomics, Microbiology, Nutrition, Obesity, Polymerization reactions, Proteomics, Respiratory, Sample preparations, Separation, Sequencing

Related Content

Antibiotics Selection Guide

The Antibiotic Selection Guide enables researchers to spend more time on their research discoveries and less time seeking established antibiotic information. Link to hundreds of antibiotics for conta...
Keywords: Antibiotics, Cell biology, Cell culture, Genetic, High performance liquid chromatography, Reversed-phase chromatography

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?