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11175041910 Roche

DIG Nucleic Acid Detection Kit

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Properties

Related Categories 12 Principles Aligned Products, Chemical Synthesis, DIG System Kits, Greener Alternative Products, Greener Life Science Products,
Quality Level   100
usage   sufficient for 40 blots (10 cm x 10 cm each)
mfr. no.   Roche
greener alternative product characteristics   Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
shipped in   dry ice
storage temp.   −20°C

Description

General description

DIG Nucleic Acid Detection Kit is used for detection of digoxigenin-labeled nucleic acids by enzyme immunoassay and enzyme-catalyzed color reaction. DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP (alkaline phosphatase) antibody conjugate and the color substrates NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate).

DIG Nucleic Acid Detection Kit is used for the detection of digoxigenin-labeled nucleic acids by enzyme immunoassay and enzyme-catalyzed color reaction. DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP (alkaline phosphatase) antibody conjugate and the color substrates NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate).

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DIG nucleic acid detection kit has been used in the confirmation of genetic manipulation.

The DIG Nucleic Acid Detection Kit contains all essential reagents for the detection of membrane-blotted, digoxigenin-labeled nucleic acid hybrids. It can be used in a variety of hybridization techniques:
• Southern blots
• Northern blots
• Other nucleic acid blotting applications
• In situ hybridization applications

The kit contains all essential reagents for the detection of membrane-blotted, digoxigenin-labeled nucleic acid hybrids. It can be used in a variety of hybridization techniques:
• Southern blots
• Northern blots
• Other nucleic acid blotting applications
• In situ hybridization applications

Features and Benefits

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Packaging

1 kit containing 5 components.

Principle

DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP antibody conjugate and the color substrates NBT and BCIP.

Preparation Note

Working concentration: Flow cytometry: 2 μg/ml (0.2 μg/100 μl/106 cells); Immunohistocytochemistry: 6 μg/ml
Working concentration of conjugate depends on application and substrate.
Storage conditions (working solution): • Anti-Digoxigenin-AP Conjugate (vial 3): 2 to 8 °C, it is stable for 12 months at this temperature
• NBT/BCIP (vial 4): 2 to 8 °C, stable
Note: During shipment of the kit on dry ice, a precipitate may occur which is dissolved by briefly warming to 37 °C
• The blocking reagent (vial 5) is stable for 36 months and can be stored dry at 4 to 8 °C.
• Autoclaved stock solution can be stored for several days to a week either unopened at 15 to 25 °C or at 4 to 8 °C after opening. Alternatively, it can be stored in aliquots at -15 to -25 °C for up to 6 months.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit component only

Description

Product #

Add to Cart

DIG-labeled Control DNA 5 µg/ml    
DNA Dilution Buffer, [50 μg/ml] fish sperm DNA    
Anti-Digoxigenin-AP Conjugate antibody 750 U/ml    
NBT/BCIP, 50x stock solution 50x concentrated    
Blocking Reagent    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Digoxigenin (DIG) Methods and Kits

The DIG System is the nonradioactive technology of choice to label and detect nucleic acids for multiple applications. The system is based on a steroid isolated from digitalis plants (Digitalis purpu...
Keywords: Amplification, Cloning, Diagnostic, Electrophoresis, Enzyme-linked immunosorbent assay, Gas chromatography, Gel electrophoresis, Immobilization, In Situ hybridization, Northern blot, Nucleic acid hybridization, Polymerase chain reaction, Purification, Transcription

Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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