Discovery Zr Carbon HPLC Columns

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Ordering Information

Discovery Zr-Carbon HPLC columns
Discovery Zr-Carbon consists of spherical, porous carbon-coated zirconia particles. It is ideal for the reversed-phase separation of positional isomers, diastereomers, and many other compounds. It complements the selectivity offering of the other zirconia-, silica-, and polymer-based Discovery phases, and allows the use of the full range of mobile phase pH from pH 1 to 14. It is a great alternative when C18 does not work.
Particle:   Zirconia (ZrO2)
Bonded Phase:   Carbon
Particle Shape:   Spherical
Particle Size:   3 and 5µm
Pore Size:   300Å
Surface Area:   30m2/g
pH Range:   1 to 14
Temperature Range:   up to 100°C in standard hardware (up to 200°C in custom all-steel hardware)
Unique retention and selectivity for method development at pH and temperature extremes
Very different selectivity than any other RP material makes it good for orthogonal screening
Excellent separation of geometric isomers and diastereomers
Very hydrophobic surface
Most different retention compared to other Discovery Zr phases for non-ionic compounds
Similar to porous graphitic carbon, but with added ion-exchange interactions
pH stable from 1-14
Thermally stable up to 200°C
Avoid fused-ring aromatics as they are too strongly absorbed by Discovery Zr-Carbon

Here's the
Carbon-based packings have found a niche within the population of HPLC supports. The main benefits of carbon over silica are enhanced chemical and thermal stability, and the ability to separate positional isomers. Compounds that have the same hydrophobicity, but different molecular shape, can be separated on the rigid carbon surface but not on phases that contain flexible ligands, like C18-bonded silica. In the figure below, the analogs ethylbenzene and p-xylene co-elute on the non-carbon Discovey Zr-PBD phase, but are resolved on Discovery Zr-Carbon.
figure O
The ability of Discovery Zr to distinguish positional isomers is demonstrated in the figure below. The isomers co-elute on a C18-silica column, but are resolved on the Discovery Zr-Carbon column.
figure P
The figure below shows a selection of twenty three different non-ionic probes. Each was run on the four Discovery Zr phases. Retention relative to benzene was plotted. For these compounds, the Discovery Zr-Carbon has the most unique selectivity.
figure Q

Ordering Information
Phase ID (mm) Length (cm) Cat. No.
3µm Discovery Zr-Carbon Columns
  2.1 5 65725-U
  2.1 7.5 65726-U
  2.1 15 65727-U
  4.6 5 65728-U
  4.6 7.5 65729-U
  4.6 15 65730-U
5µm Discovery Zr-Carbon Columns
  2.1 5 65731-U
  2.1 15 65732-U
  4.6 5 65734-U
  4.6 15 65735-U

Description 2.1mm ID 4.0mm ID
1cm Supelguard Cartridges with Discovery Zr-Carbon Packings
  3µm Particle Size
  2 Pack
65822-U 65824-U
  Kit triangle
65821-U 65823-U
  5µm Particle Size
  2 Pack
65827-U 65829-U
  Kit triangle
65826-U 65828-U
For 4.0mm ID or 4.6mm ID analytical columns
Kit includes a holder and one replaceable cartridge.

For additional information, click the pdf icons below.
view the pdf document Bulletin 932 - Discovery Zr: Method Development Guidelines (48 KB)
view the pdf documentCare and Use Guide for Discovery Zr-Carbon (15KB)

How to Contact Supelco
Phone: 800-247-6628
  or 814-359-3441
fax: 800-325-5052
email: techservice@sial.com
Outside the USA: click here

Discovery, and Supelguard are trademarks of Sigma-Aldrich Co.