Webinar: Live Cell Imaging - Novel Probes and Imaging Reagents

Date: 9th December 2020
Time: 16.00 CET
Duration: 1 hour

 What Does it Cover?

Imaging of live cells and tissues is a rapidly growing field and has revolutionized how biologists study cells, proteins and a multitude of processes and molecular interactions. Live cell imaging techniques allow scientists to observe cell structures, study dynamic processes and the localisation of molecules in real time, and over time. The observation of dynamic changes provides more insight into the operations of a cell than a snapshot provided by imaging fixed cells. Moreover, the spatial resolution of light microscopy allows observing subcellular structures and cellular processes, by using a range of fluorescent probes. Hence, live cell probes are being used instead of antibodies more frequently. In addition, since live cell imaging is less prone to experimental artifacts, it usually provides more reliable and relevant information than fixed cell microscopy.

 What Will You Learn About?

  • The new live cell probes for cell structures, reactive ions, endocytosis, exocytosis, hypoxia, nucleotide transporters, and more.
  • Probes for cellular stress by tracking reactive oxygen and nitrogen species in real-time.
  • The benefits of imaging live cells as compared to fixed cells.
  • Development of new molecular transporter that is capable of delivering NTPs across cell membranes.

Speaker Bio
Tomáš Kraus, Ph.D.
Senior researcher, Institute of Organic Chemistry and Biochemistry AS CR, Czech Republic

Dr. Tomáš Kraus has a Ph.D. from the Institute of Organic Chemistry and Biochemistry, Prague and the Institute of Chemical Technology, Prague, Czech Republic. He completed his postdoctoral studies with Jean-Pierre Sauvage, Université Louis Pasteur, Strasbourg; Individual Marie-Curie Fellowship.

In 2007 he joined the Institute of Organic Chemistry and Biochemistry CAS, Prague as a leader of the junior research group. From 2012 he took on the lead role for the "Targeted research team" at  Institute of Organic Chemistry and Biochemistry CAS, Prague, focused on the development of delivery systems for nucleoside triphosphates. He has been a Senior Researcher in the medicinal chemistry group of Prof. Michal Hocek since 2016.

Speaker Bio
Mahesh Dodla, Ph.D.
Product Manager, Cellular Assays Portfolio, MilliporeSigma, USA

Dr. Mahesh Dodla has a B. Tech. in Biotechnology from the Indian Institute of Technology, Kharagpur, India, and a Ph.D. in Biomedical Engineering from Georgia Institute of Technology, Atlanta, USA. Dr. Dodla did his postdoctoral research in human stem cell culture and neuronal differentiation at the University of Georgia, Athens, USA. Later, he joined MilliporeSigma as a Senior Scientist, editing human induced pluripotent stem cells using ZFNs and CRISPR technologies. Dr. Dodla is now part of the marketing team and has experience in managing innovation pipeline for multiple portfolios, including engineered cell lines, cell line engineering services and cell-based assays.